Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139.
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
Cold Spring Harb Perspect Med. 2018 Feb 1;8(2):a025718. doi: 10.1101/cshperspect.a025718.
Over the past decades, many approaches have been developed to fabricate biomimetic extracellular matrices of desired properties for engineering functional tissues. However, the inability of these techniques to precisely control the spatial architecture has posed a significant challenge in producing complex tissues. 3D bioprinting technology has emerged as a potential solution by bringing unprecedented freedom and versatility in depositing biological materials and cells in a well-controlled manner in the 3D volumes, therefore achieving precision engineering of functional tissues. In this article, we review the application of 3D bioprinting to tissue engineering. We first discuss the general strategies for printing functional tissue constructs. We next describe different types of bioprinting with a focus on nozzle-based techniques and their respective advantages. Finally, we summarize the limitations of current technologies and propose challenges for future development of bioprinting.
在过去的几十年中，已经开发出许多方法来制造具有所需性能的仿生细胞外基质，以用于工程功能组织。然而，这些技术无法精确控制空间结构，这在制造复杂组织方面构成了重大挑战。3D 生物打印技术的出现为解决这一问题提供了一种潜在的解决方案，它在以可控的方式在 3D 体积中沉积生物材料和细胞方面带来了前所未有的自由度和多功能性，从而实现了功能组织的精确工程。本文综述了 3D 生物打印在组织工程中的应用。我们首先讨论了打印功能组织构建体的一般策略。接下来，我们描述了不同类型的生物打印技术，重点介绍基于喷嘴的技术及其各自的优点。最后，我们总结了当前技术的局限性，并为生物打印的未来发展提出了挑战。
