Telakowski-Hopkins C A, King R G, Pickett C B
Department of Molecular Pharmacology and Biochemistry, Merck Sharp & Dohme Research Laboratories, Rahway, NJ 07065.
Proc Natl Acad Sci U S A. 1988 Feb;85(4):1000-4. doi: 10.1073/pnas.85.4.1000.
The function of the 5'-flanking region of a rat glutathione S-transferase Ya subunit structural gene has been examined in homologous and heterologous cells. By using the 5'-flanking region of the Ya subunit gene fused to the structural gene encoding chloramphenicol acetyltransferase, we have identified two cis-acting regulatory elements in the upstream region of the Ya gene. One element is required for maximum basal level expression in homologous cells, whereas maximum basal level expression in homologous cells, whereas the second element is required for inducible expression of the Ya gene by planar aromatic compounds such as beta-naphthoflavone. The cis-acting element required for inducible expression of the Ya gene by beta-naphthoflavone is functional only in cells with normal dioxin receptors.
已在同源和异源细胞中研究了大鼠谷胱甘肽S-转移酶Ya亚基结构基因5'-侧翼区的功能。通过使用与编码氯霉素乙酰转移酶的结构基因融合的Ya亚基基因的5'-侧翼区，我们在Ya基因的上游区域鉴定出两个顺式作用调节元件。一个元件是同源细胞中最大基础水平表达所必需的，而第二个元件是Ya基因被诸如β-萘黄酮等平面芳香族化合物诱导表达所必需的。β-萘黄酮诱导Ya基因表达所需的顺式作用元件仅在具有正常二噁英受体的细胞中起作用。
