Wang Chonglong, Pfleger Brian F, Kim Seon-Won
School of Biology and Basic Medical Sciences, Soochow University, Suzhou, People's Republic of China.
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI, USA.
Curr Opin Biotechnol. 2017 Jun;45:92-103. doi: 10.1016/j.copbio.2017.02.010. Epub 2017 Mar 11.
Via metabolic engineering, industrial microorganisms have the potential to convert renewable substrates into a wide range of biofuels that can address energy security and environmental challenges associated with current fossil fuels. The user-friendly bacterium, Escherichia coli, remains one of the most frequently used hosts for demonstrating production of biofuel candidates including alcohol-, fatty acid- and terpenoid-based biofuels. In this review, we summarize the metabolic pathways for synthesis of these biofuels and assess enabling technologies that assist in regulating biofuel synthesis pathways and rapidly assembling novel E. coli strains. These advances maintain E. coli's position as a prominent host for developing cell factories for biofuel production.
通过代谢工程，工业微生物有潜力将可再生底物转化为多种生物燃料，从而应对与当前化石燃料相关的能源安全和环境挑战。用户友好型细菌大肠杆菌仍然是最常用的宿主之一，用于展示生物燃料候选物的生产，包括基于酒精、脂肪酸和萜类的生物燃料。在本综述中，我们总结了这些生物燃料合成的代谢途径，并评估了有助于调节生物燃料合成途径和快速组装新型大肠杆菌菌株的使能技术。这些进展使大肠杆菌作为开发生物燃料生产细胞工厂的重要宿主的地位得以保持。
