Assih Maléki, Feteke Lochina, Bisseye Cyrille, Ouermi Djeneba, Djigma Florencia, Karou Simplice Damintoti, Simpore Jacques
Center for Biomolecular Research Pietro Annigoni, CERBA/LABIOGENE, University of Ouagadougou, Burkina Faso.
National Center for Blood Transfusion (CNTS), Lomé, Togo.
Pan Afr Med J. 2016 Dec 20;25:242. doi: 10.11604/pamj.2016.25.242.7096. eCollection 2016.
This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo. A total of 440 blood samplesfrom volunteer were collected and tested by ELISA and multiplex PCR for HBV, HCV and HIV detection. Among the 440 volunteer blood donors, 83% were female and 17% were male. Age range of 20-29 years was more represented (73%). Whereas, multiplex PCR detected more cases of HBV than ELISA (50% vs 33%, P=0.0155);ELISA more detected HCV than PCR (34% vs 3%, P<0.0001) and HIV (26% vs 7%, P<0.0001). Confirming these observations our data showed that multiplex PCR was more sensitive in the detection of HBV. The sensitivity of ELISA for the detection of HCV and HIV was elevated compared to multiplex PCR. Multiplex PCR was more specific that ELISA for the detection of HCV and HIV.Interestingly, our data showed that the gender do not influenced the sensitivity of either ELISA or multiplex PCR to detect these viruses. This study showed the limit of both ELISA and multiplex PCR in the detection of HBV, HCV and HIV.
本研究旨在比较多重聚合酶链反应（multiplex PCR）与酶联免疫吸附测定（ELISA）技术在多哥国家输血中心献血者血液样本中即时检测乙肝病毒（HBV）、丙肝病毒（HCV）和人类免疫缺陷病毒（HIV）的敏感性。共收集了440份志愿者血液样本，通过ELISA和多重PCR检测HBV、HCV和HIV。在440名志愿者献血者中，83%为女性，17%为男性。年龄在20 - 29岁的人群占比更高（73%）。然而，多重PCR检测出的HBV病例比ELISA更多（50%对33%，P = 0.0155）；ELISA检测出的HCV比PCR更多（34%对3%，P < 0.0001），HIV也是如此（26%对7%，P < 0.0001）。证实这些观察结果的数据表明，多重PCR在检测HBV方面更敏感。与多重PCR相比，ELISA检测HCV和HIV的敏感性更高。在检测HCV和HIV方面，多重PCR比ELISA更具特异性。有趣的是，我们的数据表明性别不会影响ELISA或多重PCR检测这些病毒的敏感性。本研究显示了ELISA和多重PCR在检测HBV、HCV和HIV方面的局限性。
