Ardizzoni S C, Michaels A, Arendash G W
Department of Biology, University of South Florida, Tampa 33620.
Science. 1988 Feb 5;239(4840):635-7. doi: 10.1126/science.2829355.
Gold-filled Sendai virus envelopes were fused with cell suspensions from the basal forebrain of fetal rat donors, and the resulting gold-labeled cells were transplanted into the neocortex of adult rat recipients. Not only did large numbers of labeled cells remain intact through 3 months in the neocortex, but sizable numbers migrated subcortically to the recipient's lesioned nucleus basalis region (a distance of 4 to 5 millimeters). Since this technique is capable of labeling most transplanted cells for long periods of time, it may be useful in determining the survival, migration, and connectivity of intracerebrally transplanted tissues.
将填有金颗粒的仙台病毒包膜与来自胎鼠供体基底前脑的细胞悬液融合，然后将产生的金标记细胞移植到成年大鼠受体的新皮层中。不仅大量标记细胞在新皮层中完整存活了3个月，而且相当数量的细胞还向皮层下迁移到受体受损的基底核区域（距离为4至5毫米）。由于该技术能够长时间标记大多数移植细胞，因此它可能有助于确定脑内移植组织的存活、迁移和连接情况。
