Zhang Runfeng, Li Ruiwen, Lin Yaqiu
College of Life Sciences, Hubei Normal University, Huangshi, 435002, China.
Reproductive and Endocrine Laboratory, Chengdu Woman-Child Central Hospital, Chengdu, 610051, China.
Fish Physiol Biochem. 2017 Aug;43(4):1055-1064. doi: 10.1007/s10695-017-0352-5. Epub 2017 Mar 14.
MicroRNAs (miRNAs) regulate gene expression by fully or partially binding to complementary sequences and play important roles in skeletal muscle development. However, the roles of miRNAs in the skeletal muscle of Schizothorax prenanti remain unknown. In this study, we analyzed the miRNA profiling in the skeletal muscle of S. prenanti at 30 days post-hatching (dph), 1 year, and 3 years by high-throughput sequencing. Two hundred twenty-nine unique miRNA types aligned to 201 independent pre-miRNA loci according to sequence similarity and 28 novel miRNAs were obtained. The nucleotide bias analysis of identified miRNAs showed that the miRNAs in S. prenanti were highly conserved. Eight identified miRNAs validated using stem-loop qRT-PCR were differentially expressed in the process of skeletal muscle development of S. prenanti. This study provide not only an overall insight into the miRNA landscape in the skeletal muscle of S. prenanti but also a basis for further investigation of miRNA roles in skeletal muscle development of S. prenanti.
微小RNA（miRNA）通过与互补序列完全或部分结合来调控基因表达，并在骨骼肌发育中发挥重要作用。然而，miRNA在齐口裂腹鱼骨骼肌中的作用尚不清楚。在本研究中，我们通过高通量测序分析了齐口裂腹鱼孵化后30天（dph）、1年和3年时骨骼肌中的miRNA谱。根据序列相似性，有229种独特的miRNA类型与201个独立的前体miRNA基因座比对，并获得了28个新的miRNA。对齐口裂腹鱼中已鉴定miRNA的核苷酸偏向性分析表明，这些miRNA具有高度保守性。使用茎环qRT-PCR验证的8个已鉴定miRNA在齐口裂腹鱼骨骼肌发育过程中差异表达。本研究不仅全面了解了齐口裂腹鱼骨骼肌中的miRNA情况，也为进一步研究miRNA在齐口裂腹鱼骨骼肌发育中的作用奠定了基础。
