通过结构引导的定向进化实现的酶促位点选择性。
Enzymatic site-selectivity enabled by structure-guided directed evolution.
作者信息
Wang Jian-Bo, Li Guangyue, Reetz Manfred T
机构信息
Department of Chemistry, Philipps-University Marburg, Hans-Meerwein Strasse 4, 35032, Marburg, Germany and Max-Plank-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim, Germany.
出版信息
Chem Commun (Camb). 2017 Apr 4;53(28):3916-3928. doi: 10.1039/c7cc00368d.
Biocatalytic site-selective (regioselective) organic transformations have been practiced for decades, but the traditional limitations of enzymes regarding narrow substrate acceptance and the often observed insufficient degree of selectivity have persisted until recently. With the advent of directed evolution, it is possible to engineer site-selectivity to suit the needs of organic chemists. This review features recent progress in this exciting research area, selected examples involving P450 monooxygenases, halogenases and Baeyer-Villiger monooxygenases being featured for illustrative purposes. The complementary nature of enzymes and man-made catalysts is emphasized.
生物催化的位点选择性（区域选择性）有机转化已经实践了数十年，但直到最近，酶在底物接受范围狭窄以及常常观察到的选择性程度不足等方面的传统局限性依然存在。随着定向进化的出现，有可能对位点选择性进行改造以满足有机化学家的需求。本综述介绍了这一令人兴奋的研究领域的最新进展，为说明起见，选取了涉及细胞色素P450单加氧酶、卤化酶和拜耳-维利格单加氧酶的实例。文中强调了酶与人造催化剂的互补性质。
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