Bolvig Anne K, Nørskov Natalja P, Hedemann Mette S, Foldager Leslie, McCarthy-Sinclair Brendan, Marco Maria L, Lærke Helle N, Bach Knudsen Knud E
Department of Animal Science, Faculty of Science and Technology, Aarhus University , Blichers Allé 20, PO Box 50, DK-8830 Tjele, Denmark.
Bioinformatics Research Centre, Faculty of Science and Technology, Aarhus University , C.F. Møllers Allé 8, DK-8000 Aarhus C, Denmark.
J Proteome Res. 2017 Jun 2;16(6):2135-2150. doi: 10.1021/acs.jproteome.6b00942. Epub 2017 May 3.
High plant lignan intake is associated with a number of health benefits, possibly induced by the lignan metabolite enterolactone (ENL). The gut microbiota plays a crucial role in converting dietary lignans into ENL, and epidemiological studies have shown that use of antibiotics is associated with lower levels of ENL. Here we investigate the link between antibiotic use and lignan metabolism in pigs using LC-MS/MS. The effect of lignan intake and antibiotic use on the gut microbial community and the pig metabolome is studied by 16S rRNA sequencing and nontargeted LC-MS. Treatment with antibiotics resulted in substantially lower concentrations of ENL compared with concentrations detected in untreated animals, whereas the plasma concentrations of plant lignans were unchanged. Both diet and antibiotic treatment affected the clustering of urinary metabolites and significantly altered the proportions of taxa in the gut microbiota. Diet, but not antibiotic treatment, affected the plasma lipid profile, and a lower concentration of LDL cholesterol was observed in the pigs fed a high lignan diet. This study provides solid support for the associations between ENL concentrations and use of antibiotics found in humans and indicates that the lower ENL concentration may be a consequence of the ecological changes in the microbiota.
高植物木脂素摄入量与多种健康益处相关，可能是由木脂素代谢产物肠内酯（ENL）引起的。肠道微生物群在将膳食木脂素转化为ENL的过程中起着关键作用，流行病学研究表明，使用抗生素与较低水平的ENL有关。在这里，我们使用液相色谱-串联质谱法（LC-MS/MS）研究猪使用抗生素与木脂素代谢之间的联系。通过16S rRNA测序和非靶向液相色谱-质谱法研究木脂素摄入量和抗生素使用对肠道微生物群落和猪代谢组的影响。与未治疗动物中检测到的浓度相比，抗生素治疗导致ENL浓度大幅降低，而植物木脂素的血浆浓度没有变化。饮食和抗生素治疗均影响尿液代谢物的聚类，并显著改变肠道微生物群中分类群的比例。饮食而非抗生素治疗影响血浆脂质谱，在喂食高木脂素饮食的猪中观察到较低浓度的低密度脂蛋白胆固醇。这项研究为人类中发现的ENL浓度与抗生素使用之间的关联提供了有力支持，并表明较低的ENL浓度可能是微生物群生态变化的结果。
