In Gyo, Seo Hyun Kyu, Park Hee-Won, Jang Kyoung Hwa
Korea Ginseng Research Institute, Korea Ginseng Corporation, Daejeon 305-805, Korea.
Molecules. 2017 Mar 15;22(3):471. doi: 10.3390/molecules22030471.
Ginsenosides are used as existing markers of red ginseng (RG) quality, and ginsenoside ratios are also indicative of the different components of red ginseng. For the analysis and classification of ginsenoside content, red ginseng was separated into three parts, namely, main roots, lateral roots, and fine roots, and each extract was subjected to ultra-performance liquid chromatography quadruple time-of-flight mass spectrometry (UPLC-QToF-MS) with multivariate statistical analysis. Principal component analysis (PCA) showed a clear discrimination between the extracts of main roots and fine roots and suggested discrimination markers (four for the main roots and five for the fine roots). The fine root markers were identified as ginsenoside. We identified two markers for the main roots of red ginseng in this study. Moreover, the contents of 22 ginsenosides were analyzed in all three components of red ginseng. Fine roots have the highest protopanaxadiol (PPD)/protopanaxatriol (PPT) ratio. The PPD group of ginsenosides, which is quantitatively dominant in fine roots, clearly distinguishes the main roots from the other parts.
人参皂苷被用作红参（RG）质量的现有标志物，人参皂苷比例也表明了红参的不同成分。为了分析和分类人参皂苷含量，将红参分为三个部分，即主根、侧根和细根，每种提取物都进行了超高效液相色谱四极杆飞行时间质谱（UPLC-QToF-MS）分析，并进行多变量统计分析。主成分分析（PCA）显示主根和细根提取物之间有明显的区分，并提出了区分标志物（主根有4个，细根有5个）。细根标志物被鉴定为人参皂苷。在本研究中，我们鉴定出了红参主根的两个标志物。此外，还分析了红参所有三个部分中22种人参皂苷的含量。细根的原人参二醇（PPD）/原人参三醇（PPT）比例最高。在细根中定量占主导地位的PPD组人参皂苷明显将主根与其他部分区分开来。
