Marriott Clare L, Dutton Emma E, Tomura Michio, Withers David R
Institute of Immunology & Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, UK.
Laboratory of Immunology, Faculty of Pharmacy, Osaka-Ohtani University 3-11-1 Nishikiorikita, Tondabayashi-city, Osaka prefecture, Japan.
Eur J Immunol. 2017 May;47(5):860-871. doi: 10.1002/eji.201646681. Epub 2017 Apr 11.
Several different memory T-cell populations have now been described based upon surface receptor expression and migratory capabilities. Here we have assessed murine endogenous memory CD4 T cells generated within a draining lymph node and their subsequent migration to other secondary lymphoid tissues. Having established a model response targeting a specific peripheral lymph node, we temporally labelled all the cells within draining lymph node using photoconversion. Tracking of photoconverted and non-photoconverted Ag-specific CD4 T cells revealed the rapid establishment of a circulating memory population in all lymph nodes within days of immunisation. Strikingly, a resident memory CD4 T cell population became established in the draining lymph node and persisted for several months in the absence of detectable migration to other lymphoid tissue. These cells most closely resembled effector memory T cells, usually associated with circulation through non-lymphoid tissue, but here, these cells were retained in the draining lymph node. These data indicate that lymphoid tissue resident memory CD4 T-cell populations are generated in peripheral lymph nodes following immunisation.
现在已经根据表面受体表达和迁移能力描述了几种不同的记忆性T细胞群体。在此，我们评估了引流淋巴结内产生的小鼠内源性记忆性CD4 T细胞及其随后向其他二级淋巴组织的迁移。在建立了针对特定外周淋巴结的模型反应后，我们使用光转化对引流淋巴结内的所有细胞进行了时间标记。对光转化和未光转化的抗原特异性CD4 T细胞的追踪显示，在免疫后数天内，所有淋巴结中迅速建立了循环记忆群体。引人注目的是，引流淋巴结中建立了驻留记忆性CD4 T细胞群体，并且在没有检测到向其他淋巴组织迁移的情况下持续了几个月。这些细胞与效应记忆T细胞最为相似，效应记忆T细胞通常与通过非淋巴组织的循环有关，但在这里，这些细胞保留在引流淋巴结中。这些数据表明，免疫后外周淋巴结中会产生淋巴组织驻留记忆性CD4 T细胞群体。
