Chen Yue, Wang Yunzhu, Lyu Ping, Chen Liping, Shen Chenjia, Sun Chongbo
Institute of Horticulture, Zhejiang Academy of Agriculture Science, Hangzhou, Zhejiang, People's Republic of China.
Key Laboratory of Creative Agriculture, Ministry of Agriculture, Hangzhou, People's Republic of China.
J Plant Res. 2019 May;132(3):419-429. doi: 10.1007/s10265-019-01099-6. Epub 2019 Mar 22.
Dendrobium officinale is a traditional medicinal herb with a variety of bioactive components. Alkaloid is one of the major active ingredients of Dendrobium plants, and its immune regulatory effects have been well-studied. Although a number of genes involved in the biosynthetic pathway of alkaloids have been elucidated, the regulation mechanism underlying the methyl-jasmonate (MeJA)-induced accumulation of alkaloids in D. officinale is largely unknown. In our study, a total of 4,857 DEGs, including 2,943 up- and 1,932 down-regulated genes, were identified between the control and MeJA-treated groups. Kyoto Encyclopedia of Genes and Genomes annotation showed that a number of DEGs were associated with the putative alkaloid biosynthetic pathway in D. officinale. The main group of Dendrobium alkaloids are sesquiterpene alkaloids, which are the downstream products of mevalonate (MVA) and methylerythritol 4-phosphate (MEP) pathway. Several MVA and MEP pathway genes were significantly up-regulated by the MeJA treatment, suggesting an active precursor supply for the alkaloid biosynthesis under MeJA treatment. A number of MeJA-induced P450 family genes, aminotransferase genes and methyltransferase genes were identified, providing several important candidates to further elucidate the sesquiterpene alkaloid biosynthetic pathway of D. officinale. Furthermore, a large number of MeJA-induced transcript factor encoding genes were identified, suggesting a complex genetic network affecting the sesquiterpene alkaloid metabolism in D. officinale. Our data aids to reveal the regulation mechanism underlying the MeJA-induced accumulation of sesquiterpene alkaloids in D. officinale.
铁皮石斛是一种具有多种生物活性成分的传统草药。生物碱是石斛属植物的主要活性成分之一，其免疫调节作用已得到充分研究。尽管已经阐明了许多参与生物碱生物合成途径的基因，但茉莉酸甲酯（MeJA）诱导铁皮石斛生物碱积累的调控机制仍 largely 未知。在我们的研究中，在对照组和 MeJA 处理组之间共鉴定出 4857 个差异表达基因（DEGs），其中包括 2943 个上调基因和 1932 个下调基因。京都基因与基因组百科全书注释显示，许多 DEGs 与铁皮石斛假定的生物碱生物合成途径相关。石斛生物碱的主要类别是倍半萜生物碱，它们是甲羟戊酸（MVA）和甲基赤藓糖醇 4-磷酸（MEP）途径的下游产物。MeJA 处理显著上调了几个 MVA 和 MEP 途径基因，表明在 MeJA 处理下生物碱生物合成有活跃的前体供应。鉴定出了一些 MeJA 诱导的细胞色素 P450 家族基因、氨基转移酶基因和甲基转移酶基因，为进一步阐明铁皮石斛倍半萜生物碱生物合成途径提供了几个重要候选基因。此外，还鉴定出了大量 MeJA 诱导的转录因子编码基因，表明存在一个复杂的遗传网络影响铁皮石斛倍半萜生物碱代谢。我们的数据有助于揭示 MeJA 诱导铁皮石斛倍半萜生物碱积累的调控机制。
