电化学质子梯度与呼吸作用之间的关系：丙酮酸和L-棕榈酰肉碱作为大鼠心脏线粒体的氢供体
Relationship between the electrochemical proton gradient and respiration: pyruvate and l-palmitoylcarnitine as hydrogen donors for rat heart mitochondria.
作者信息
Schönfeld P, Petzold D
机构信息
Institut für Biochemie der Medizinischen Akademie, Magdeburg.
出版信息
Biomed Biochim Acta. 1987;46(8-9):S549-52.
The electrochemical proton gradient (delta/uH+) and the rate of respiration were determined as function of the work load at conditions where isolated rat heart mitochondria utilize pyruvate and/or l-palmitoyl-carnitine as hydrogen donors. Delta/uH+ was calculated from the distribution of 86Rb+ (delta psi marker) and [14C]-dimethyloxazolidine-2,4-dione (delta pH marker) between the mitochondrial matrix space and the medium. A transition from l-palmitoylcarnitine to pyruvate utilization did not change the rate of hydrogen supply or the mitochondrial energy state.
在离体大鼠心脏线粒体利用丙酮酸和/或L-棕榈酰肉碱作为氢供体的条件下，测定了电化学质子梯度（δμH⁺）和呼吸速率与工作负荷的函数关系。δμH⁺是根据⁸⁶Rb⁺（δψ标记物）和[¹⁴C]-二甲基恶唑烷-2,4-二酮（δpH标记物）在线粒体基质空间和培养基之间的分布计算得出的。从L-棕榈酰肉碱利用向丙酮酸利用的转变并未改变氢供应速率或线粒体能量状态。
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