Petzelt Christian, Bier Karlheinz
Zoologisches Institut der Universität Münster, Deutschland.
Max-Planck-Institut für Zellbiologie Institut Tübingen, Melanchthonstr. 36, 74 Tübingen.
Wilhelm Roux Arch Entwickl Mech Org. 1970 Dec;164(4):359-366. doi: 10.1007/BF00577811.
By means of radioautographic and electrophoretic techniques yolk protein uptake in the growing oocytes ofMusca domestica was investigated. After 30 min yolk protein becomes visible in the radioautograms. By stainability and labeling the yolk fraction could be demonstrated in the pherogram of ovary and haemolymph in an intermediate developmental stage (stage 3). After the end of vitellogenesis it does not appear in the haemolymph. The yolk protein uptake is inhibited by Actinomycin, but the synthesis goes on nearly as normal. This inhibition can be interpretated as a new accessory effect of Actinomycin.
通过放射自显影和电泳技术,对家蝇生长中的卵母细胞摄取卵黄蛋白的情况进行了研究。30分钟后,放射自显影片中可见卵黄蛋白。通过染色性和标记,在中间发育阶段(第3阶段)的卵巢和血淋巴的电泳图谱中可以证实卵黄部分的存在。卵黄发生结束后,它不再出现在血淋巴中。放线菌素可抑制卵黄蛋白的摄取,但合成几乎仍正常进行。这种抑制作用可被解释为放线菌素的一种新的附加效应。