Berking Stefan
Zoologisches Institut der Universität, Im Neuenheimer Feld 230, D-6900, Heidelberg, Federal Republic of Germany.
Wilehm Roux Arch Dev Biol. 1983 Nov;192(6):327-332. doi: 10.1007/BF00848812.
A fraction has been purified from crude extracts ofHydra, termed inhibitor I, which inhibits budding as well as head and foot regeneration. This fraction has been proposed by others to contain (at least) two substances, a head inhibitor (HI) and a foot inhibitor (FI), which preferentially inhibit head and foot regeneration respectively. The present study indicates that the reported fractionation is an artefact produced by the chromatographic procedure used. Substances can be eluted from virgin Dowex 50 which preferentially inhibit head regeneration. These substances are formed in the resin if left standing in its hydrogen form. Further, we have not been able to confirm that the extract from heads preferentially inhibits head regeneration and the extract from feet preferentially inhibits foot regeneration. Rather it appears that the inhibitor I is contained both in the head and in the foot.
从水螅的粗提物中纯化出一种成分，称为抑制剂I，它能抑制出芽以及头部和足部的再生。其他人提出该成分（至少）含有两种物质，一种头部抑制剂（HI）和一种足部抑制剂（FI），它们分别优先抑制头部和足部的再生。本研究表明，报道的分级分离是所用色谱方法产生的假象。从原始的Dowex 50树脂上可以洗脱下来一些物质，它们优先抑制头部再生。如果树脂以氢型静置，这些物质会在树脂中形成。此外，我们无法证实头部提取物优先抑制头部再生，足部提取物优先抑制足部再生。相反，似乎抑制剂I同时存在于头部和足部。
