Ojo Oluwafemi Adeleke, Afon Adeola Agnes, Ojo Adebola Busola, Ajiboye Basiru Olaitan, Oyinloye Babatunji Emmanuel, Kappo Abidemi Paul
Phytomedicine, Biomedical Toxicology and Diabetes Research Laboratories, Department of Biochemistry, Afe Babalola University, Ado-Ekiti 360001, Nigeria.
Department of Biochemistry, University of Ilorin, Ilorin 240222, Nigeria.
Antioxidants (Basel). 2018 May 26;7(6):73. doi: 10.3390/antiox7060073.
Therapies directed towards controlling hyperglycemia, the hallmark of type-2 diabetes mellitus, go a long way in managing diabetes and its related complications. Reducing glucose level through the inhibition of the relevant carbohydrate hydrolyzing enzymes is one among many routes in the management of diabetes. This study investigates the enzyme inhibitory and antioxidant properties of solvent-partitioned fractions of and leaves; which are used extensively in the treatment of diabetic patients locally. The leaves of and were extracted with methanol and fractionated to obtain -hexane (HF), ethyl acetate (EAF), -butanol (BF), and aqueous (AF) fractions successively. The α-amylase and α-glucosidase inhibitory activities of fractions of and leaves were investigated while the antioxidant activity of each fraction was analyzed using iron chelating and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid) radical scavenging assay. Our findings indicated that the ethyl acetate fraction of leaves contained a considerably higher ( < 0.05) amount of total phenolic, flavonoids, metal ion, and ABTS radical scavenging activity than the ethyl acetate fractions of . Furthermore, the ethyl acetate fraction of had a considerably higher ( < 0.05) inhibitory effect on α-glucosidase (IC = 25.11 ± 0.01 μg mL), and α-amylase (IC = 24.04 ± 0.12 μg mL) activities than the fraction. Hence, the inhibitory activities of and leaves suggest that they are a potential source of orally active antidiabetic agents and could be employed to formulate new plant-based pharmaceutical and nutraceutical drugs to improve human health.
针对控制高血糖（2型糖尿病的标志）的疗法在管理糖尿病及其相关并发症方面大有帮助。通过抑制相关碳水化合物水解酶来降低血糖水平是糖尿病管理众多途径之一。本研究调查了[植物名称1]和[植物名称2]叶子的溶剂分配馏分的酶抑制和抗氧化特性；这两种植物在当地被广泛用于治疗糖尿病患者。[植物名称1]和[植物名称2]的叶子用甲醇提取，然后依次分馏得到正己烷馏分（HF）、乙酸乙酯馏分（EAF）、正丁醇馏分（BF）和水相馏分（AF）。研究了[植物名称1]和[植物名称2]叶子各馏分的α-淀粉酶和α-葡萄糖苷酶抑制活性，同时使用铁螯合和ABTS（2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸））自由基清除试验分析了每个馏分的抗氧化活性。我们的研究结果表明，[植物名称1]叶子的乙酸乙酯馏分比[植物名称2]叶子的乙酸乙酯馏分含有显著更高（P<0.05）的总酚、黄酮类、金属离子和ABTS自由基清除活性。此外，[植物名称1]叶子的乙酸乙酯馏分对α-葡萄糖苷酶（IC50 = 25.11±0.01μg/mL）和α-淀粉酶（IC50 = 24.04±0.12μg/mL）活性的抑制作用比[植物名称2]叶子的乙酸乙酯馏分显著更高（P<0.05）。因此，[植物名称1]和[植物名称2]叶子的抑制活性表明它们是口服活性抗糖尿病药物的潜在来源，可用于开发新的植物源药物和营养保健品以改善人类健康。
