Cundiff D D, Besch-Williford C, Hook R R, Franklin C L, Riley L K
Department of Veterinary Pathology, University of Missouri, Columbia 65211.
J Clin Microbiol. 1994 Aug;32(8):1930-4. doi: 10.1128/jcm.32.8.1930-1934.1994.
The cilia-associated respiratory (CAR) bacillus is an unclassified, gram-negative, motile bacterium that has been implicated as an etiologic agent of respiratory disease in laboratory rodents. In the present study, approximately 1,200 bases of the 16S rRNA gene from three CAR bacillus isolates were sequenced. CAR bacillus-specific primers were designed on the basis of the 16S rRNA gene sequence and used in a PCR assay. The PCR assay detected as little as 500 fg of purified CAR bacillus DNA. The expected 267-bp DNA fragment was amplified from respiratory tissue of frozen, formalin-fixed, and paraffin-embedded samples from experimentally and naturally infected rats and mice. In contrast, no product was amplified from respiratory tissues of sham-infected experimental animals or animals that were serologically or histopathologically negative for the CAR bacillus. Our findings indicate that this PCR assay is a rapid, specific, and sensitive detection method for the diagnosis of CAR bacillus infection in rats and mice.
纤毛相关呼吸道（CAR）杆菌是一种未分类的革兰氏阴性、运动性细菌，被认为是实验啮齿动物呼吸道疾病的病原体。在本研究中，对来自三株CAR杆菌分离株的16S rRNA基因的约1200个碱基进行了测序。基于16S rRNA基因序列设计了CAR杆菌特异性引物，并用于PCR检测。该PCR检测法能检测到低至500 fg的纯化CAR杆菌DNA。从实验性和自然感染的大鼠和小鼠的冷冻、福尔马林固定和石蜡包埋样本的呼吸道组织中扩增出预期的267 bp DNA片段。相比之下，从假感染实验动物或血清学或组织病理学检测CAR杆菌呈阴性的动物的呼吸道组织中未扩增出产物。我们的研究结果表明，这种PCR检测法是一种快速、特异且灵敏的诊断大鼠和小鼠CAR杆菌感染的检测方法。
