Leach L, Trudgill D L, Gahan P B
Department of Biology, King's College London, Kensington Campus, UK.
Histochem J. 1987 Sep;19(9):471-5. doi: 10.1007/BF01675416.
Mammalian antibodies to the neuroamines, serotonin and gamma-amino-butyric acid (GABA) and to the neuropeptides, adrenocorticotrophic hormone (ACTH) and FMRF-amide evoked a response to Goodeyus ulmi, a free-living nematode. Serotonin-like immunoreactivity was found in cell bodies in the nerve ring and in the ventral nerve cord in all developmental stages. Neurons in the vulva, implicated in egg-laying, were immunoreactive to anti-serotonin in G. ulmi females, while in males serotonergic nerve fibres was found in the spicular region. Immunoreactivity to ACTH was also seen to differ depending on the developmental stage of G. ulmi, being present only in the ventral cord from the late L3 stage. Anti-GABA immunoreactivity was localized in two cell bodies near the amphids in all life stages and FMRF-amide immunoreactivity was seen in the nerve ring in all developmental stages. No reactivity was found with antibodies to vasointestinal peptide and somatostatin-14.
针对神经胺、5-羟色胺和γ-氨基丁酸（GABA）以及神经肽、促肾上腺皮质激素（ACTH）和FMRF酰胺的哺乳动物抗体，对自由生活的线虫古德伊线虫（Goodeyus ulmi）产生了反应。在所有发育阶段，神经环和腹神经索的细胞体中均发现了5-羟色胺样免疫反应性。在外阴中与产卵有关的神经元，在古德伊线虫雌性中对抗5-羟色胺具有免疫反应性，而在雄性中，在交合刺区域发现了5-羟色胺能神经纤维。对ACTH的免疫反应性也因古德伊线虫的发育阶段而异，仅在L3晚期的腹索中存在。抗GABA免疫反应性在所有生命阶段均定位于近头感器的两个细胞体中，并且在所有发育阶段的神经环中均可见FMRF酰胺免疫反应性。未发现与血管活性肠肽和生长抑素-14抗体的反应性。
