Zhang Lei, Zhao Mingkui, Sui Ru-Bo
School of Nursing, Jinzhou Medical University, Jinzhou, China.
Department of Neurology, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.
Cell Physiol Biochem. 2017;41(4):1403-1412. doi: 10.1159/000467940. Epub 2017 Mar 16.
To identify the molecular mechanism of post-stroke depression (PSD), and observe the therapeutic effects of cerebellar fastigial nucleus electrical stimulation (FNS) on the behaviors and regional cerebral blood flow (rCBF) in a PSD rat model.
Healthy SD rats were randomly divided into four groups (sham, stroke, post-stroke depress and FNS group). Sham group (n = 6) underwent sham operation. The other three groups (n = 6*3) underwent MCAO. Rats were examined twice a week in open filed test. Moreover, neuroprotective effect on cerebellar Purkinje cells and expression of cytokines in hippocampal tissue were examined.
The PSD group showed a significant weight loss, decreased consumption of sucrose water, reduced rearing and locomotor activities. The FNS significantly alleviates the body weight loss and sucrose preference, locomotor and rearing activities. The bilateral rCBF was also restored after FNS treatment. Moreover, FNS improved the neuroprotection via suppressing apoptosis of cerebellar Purkinje cells. And the inflammatory cytokines mRNA level in hippocampus was significantly decreased.
FNS treatment alleviates depressive-like behaviors and rCBF in PSD rats model, which could be attributed to its ability to protect cerebellar Purkinje cells and decrease the mRNA level of inflammatory cytokines.
确定脑卒中后抑郁（PSD）的分子机制，并观察小脑顶核电刺激（FNS）对PSD大鼠模型行为和局部脑血流量（rCBF）的治疗效果。
将健康的SD大鼠随机分为四组（假手术组、脑卒中组、脑卒中后抑郁组和FNS组）。假手术组（n = 6）进行假手术。其他三组（n = 6×3）进行大脑中动脉闭塞（MCAO）手术。每周两次在旷场试验中对大鼠进行检测。此外，检测对小脑浦肯野细胞的神经保护作用以及海马组织中细胞因子的表达。
PSD组体重显著减轻，糖水消耗量减少，直立和运动活动减少。FNS显著减轻体重减轻和蔗糖偏好、运动和直立活动。FNS治疗后双侧rCBF也恢复。此外，FNS通过抑制小脑浦肯野细胞凋亡改善神经保护作用。海马中炎性细胞因子mRNA水平显著降低。
FNS治疗可减轻PSD大鼠模型中的抑郁样行为和rCBF，这可能归因于其保护小脑浦肯野细胞和降低炎性细胞因子mRNA水平的能力。
