Anilir Ender, Ozen Filiz, Yildirim Ibrahim Halil, Ozemir Ibrahim Ali, Ozlu Can, Alimoglu Orhan
Department of General Surgery, American Hospital of İstanbul, Turkey.
Medical Genetic Department, İstanbul Medeniyet University, Turkey.
Ann Hepatobiliary Pancreat Surg. 2017 Feb;21(1):30-38. doi: 10.14701/ahbps.2017.21.1.30. Epub 2017 Feb 28.
BACKGROUNDS/AIMS: Inflammatory mediators of the innate immune response play fundamental roles in the pathogenesis of acute pancreatitis. The correlation between interleukin-8 (IL-8) gene polymorphism with types of acute pancreatitis and severity of pancreatitis, was evaluated in this study.
According to the diagnostic criteria, 176 patients with acute pancreatitis were grouped into biliary (n=83) and nonbiliary pancreatitis (n=93). Healthy blood donors (n=100) served as controls. Serum alanine transaminase, aspartate transaminase, total and direct bilirubin, amylase, lypase, white blood cell count and c-reactive protein levels were evaluated to correlate with IL-8 rs4073 (-251T/A) polymorphism, which was analyzed using a real-time polymerase chain reaction method with melting point analysis.
The IL-8 AA genotype was detected with a significantly higher frequency among the patients with acute biliary pancreatitis having higher alanine transaminase levels than the median range. Homozygote alleles were significantly higher among patients with acute biliary pancreatitis having amylase levels higher than the median range.
Determination of the frequency of IL-8 polymorphism in acute pancreatitis is informative and provides further evidence concerning the role of IL-8 in laboratory tests.
背景/目的：固有免疫反应的炎症介质在急性胰腺炎的发病机制中起重要作用。本研究评估了白细胞介素-8（IL-8）基因多态性与急性胰腺炎类型及胰腺炎严重程度之间的相关性。
根据诊断标准，将176例急性胰腺炎患者分为胆源性胰腺炎组（n = 83）和非胆源性胰腺炎组（n = 93）。100名健康献血者作为对照。评估血清丙氨酸转氨酶、天冬氨酸转氨酶、总胆红素和直接胆红素、淀粉酶、脂肪酶、白细胞计数及C反应蛋白水平，以与IL-8 rs4073（-251T/A）多态性相关联，采用实时聚合酶链反应法及熔解曲线分析对其进行分析。
在丙氨酸转氨酶水平高于中位数范围的急性胆源性胰腺炎患者中，检测到IL-8 AA基因型的频率显著更高。在淀粉酶水平高于中位数范围的急性胆源性胰腺炎患者中，纯合子等位基因显著更高。
测定急性胰腺炎中IL-8多态性的频率具有参考价值，并为IL-8在实验室检查中的作用提供了进一步的证据。
