Yang Tao, Groenewald Johannes Z, Cheewangkoon Ratchadawan, Jami Fahimeh, Abdollahzadeh Jafar, Lombard Lorenzo, Crous Pedro W
CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
Fungal Biol. 2017 Apr;121(4):322-346. doi: 10.1016/j.funbio.2016.11.001. Epub 2016 Nov 21.
Members of Botryosphaeriales are ecologically diverse, but most commonly associated with leaf spots, fruit and root rots, die-back or cankers of diverse woody hosts. Based on morphology and DNA sequence data, the Botryosphaeriales have to date been shown to contain eight families, with an additional two, Endomelanconiopsisaceae (Endomelanconiopsis) and Pseudofusicoccumaceae (Pseudofusicoccum) being newly described in this study. Furthermore, Oblongocollomyces is introduced as new genus, while Spencermartinsia is reduced to synonymy under Dothiorella. Novel species include Diplodia pyri (Pyrus sp., the Netherlands), Diplodia citricarpa (Citrus sp., Iran), Lasiodiplodia vitis (Vitis vinifera, Italy), L. sterculiae (Sterculia oblonga, Germany), Neofusicoccum pistaciarum (Pistacia vera, USA), N. buxi (Buxus sempervirens, France), N. stellenboschiana (Vitis vinifera, South Africa), and Saccharata hawaiiensis (Protea laurifolia, Hawaii). New combinations are also proposed for Camarosporium pistaciae (associated with fruit rot of Pistacia vera) in Neofusicoccum, and Sphaeria gallae (associated with galls of Quercus) in Diplodia. The combination of large subunit of the nuclear ribosomal RNA gene (LSU)-rpb2 proved effective at delineating taxa at family and generic level. Furthermore, rpb2 also added additional resolution for species delimitation, in combination with ITS, tef1 and tub2. In this study we analysed 499 isolates, and produce an expanded phylogenetic backbone for Botryosphaeriales, which will help to delimit novelties at species, genus and family level in future.
葡萄座腔菌目成员的生态多样性丰富，但最常与叶斑病、果实和根腐病、梢枯或多种木本寄主的溃疡病有关。基于形态学和DNA序列数据，迄今为止已证明葡萄座腔菌目包含八个科，本研究中新描述了另外两个科，即内黑座腔菌科（内黑座腔菌属）和拟隐壳孢科（拟隐壳孢属）。此外，引入了长孢腔菌属作为新属，而斯宾塞马丁氏菌属被归为座壳孢属的异名。新物种包括梨色二孢（荷兰梨属物种）、柑桔色二孢（伊朗柑橘属物种）、葡萄色二孢（意大利酿酒葡萄）、长蒴黄麻色二孢（德国长蒴黄麻）、阿月浑子新黑腐皮壳菌（美国阿月浑子）、欧洲黄杨新黑腐皮壳菌（法国欧洲黄杨）、南非新黑腐皮壳菌（南非酿酒葡萄）和夏威夷糖孢（夏威夷香叶树）。还对新黑腐皮壳菌属中的黄连木痂圆孢（与阿月浑子果实腐烂有关）和座壳孢属中的栎树瘿座壳（与栎树瘿有关）提出了新组合。核糖体RNA基因大亚基（LSU）-rpb2的组合被证明在科级和属级分类单元的划分中有效。此外，rpb2与ITS、tef1和tub2结合，在物种划分上也增加了额外的分辨率。在本研究中，我们分析了499个分离株，并为葡萄座腔菌目构建了一个扩展的系统发育主干，这将有助于在未来界定物种、属和科级别的新分类单元。
