Yang Bintong, Song Haichao, Hu Renge, Tao Luotao, Liang Zhenlin, Cong Wei, Kang Yuanhuan
Marine College, Shandong University/Key Laboratory of Modern Marine Ranching Technology of Weihai, Weihai, 264209, China.
Shandong Fu Han Ocean Sci-Tech Co., Ltd, Haiyang, 265100, China.
Probiotics Antimicrob Proteins. 2025 Feb;17(1):212-226. doi: 10.1007/s12602-023-10149-4. Epub 2023 Aug 26.
The application of probiotics, in aquaculture, is becoming increasingly widespread and have had positive application effects. However, reports of loach-derived probiotics are quite limited. In this study, two representative strains of lactic acid bacteria with excellent traits, namely, Weissella confusa N17 and Lactobacillus saniviri N19, were screened from the intestine of healthy loaches. W. confusa N17 and L. saniviri N19 could inhibit different common various pathogenic bacteria, especially Aeromonas spp., and were sensitive to the most common antibiotics. The survival rate of the two strains exceeded 50% after 4 h of incubation in 10% loach bile. Moreover, the two strains showed significant tolerance to trypsin. Their autoaggregation capacity and hydrophobicity were greater than 30%. In addition, the aggregation ability of both strains was higher than 30% for both A. veronii TH0426 and A. hydrophila TPS. The two strains had a high biofilm-forming ability and strong adhesion to epithelioma papulosum cyprini (EPC) cells. Scanning electron microscopy results showed that the culture supernatants of the two strains had a significantly destructive effect on A. veronii TH0426 and A. hydrophila TPS. Overall, the traits of W. confusa N17 were better than those of L. saniviri N19. Genome sequencing and analysis demonstrated a lack of virulence factor-related or drug resistance-related genes in genome N17. The diet supplemented with the W. confusa N17 strain significantly improved the resistance of loaches to A. veronii infection, and the protection rate reached 57.1%. Therefore, W. confusa N17 exhibits promising for further applications in loach aquaculture.
益生菌在水产养殖中的应用越来越广泛，并已产生了积极的应用效果。然而，关于泥鳅源益生菌的报道相当有限。在本研究中，从健康泥鳅的肠道中筛选出了两株具有优良特性的代表性乳酸菌菌株，即困惑魏斯氏菌N17和无害乳杆菌N19。困惑魏斯氏菌N17和无害乳杆菌N19能够抑制不同的常见病原菌，尤其是气单胞菌属，并且对最常用的抗生素敏感。在10%的泥鳅胆汁中培养4小时后，这两株菌的存活率超过50%。此外，这两株菌对胰蛋白酶表现出显著的耐受性。它们的自聚集能力和疏水性均大于30%。此外，对于维氏气单胞菌TH0426和嗜水气单胞菌TPS，这两株菌的聚集能力均高于30%。这两株菌具有较高的生物膜形成能力，并且对鲤上皮瘤细胞（EPC）有很强的黏附性。扫描电子显微镜结果表明，这两株菌的培养上清液对维氏气单胞菌TH0426和嗜水气单胞菌TPS有显著的破坏作用。总体而言，困惑魏斯氏菌N17的特性优于无害乳杆菌N19。基因组测序和分析表明，基因组N17中缺乏与毒力因子相关或耐药相关的基因。添加困惑魏斯氏菌N17菌株的饲料显著提高了泥鳅对维氏气单胞菌感染 的抵抗力，保护率达到57.1%。因此，困惑魏斯氏菌N17在泥鳅养殖中具有进一步应用的潜力。
