Jia Lu, Qiu Jichuan, Du Lingqian, Li Zhi, Liu Hong, Ge Shaohua
Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan 250012, China.
Department of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China.
Nanomedicine (Lond). 2017 Apr;12(7):761-776. doi: 10.2217/nnm-2016-0398. Epub 2017 Mar 21.
To investigate the photocatalytic inactivation of fungi and bacteria mediated by TiO nanorod arrays (TNRs).
MATERIALS & METHODS: The features of TNRs were characterized by scanning electron microscopy, atomic force microscopy, transmission electron microscopy, x-ray diffraction (XRD) and contact angle measurement. The antimicrobial efficiency was detected on biofilm and planktonic forms of Candida albicans, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis by crystal violet and XTT (2,3-bis [2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide) assay, confocal laser scanning microscope and colony-forming assay. Fluorogenic quantitative assay was used to explore the underlying mechanism.
RESULTS & CONCLUSION: TNRs were well aligned and vertically oriented on Ti with a diameter of about 100 μm, possessing a rougher surface and better hydrophilicity. Moreover, TNRs exhibited significantly higher antifungal and antibacterial efficiency compared with Ti under UV irradiation, laying the foundation for surface modification of implants with TNRs.
研究二氧化钛纳米棒阵列（TNRs）介导的真菌和细菌的光催化失活作用。
通过扫描电子显微镜、原子力显微镜、透射电子显微镜、X射线衍射（XRD）和接触角测量对TNRs的特征进行表征。通过结晶紫和XTT（2,3-双[2-甲氧基-4-硝基-5-磺基苯基]-2H-四唑-5-羧基苯胺）测定、共聚焦激光扫描显微镜和菌落形成测定，检测TNRs对白色念珠菌、伴放线聚集杆菌和牙龈卟啉单胞菌生物膜和浮游形式的抗菌效率。采用荧光定量测定法探究其潜在机制。
TNRs在钛表面排列良好且垂直取向，直径约为100μm，表面更粗糙且亲水性更好。此外，在紫外线照射下，TNRs表现出比钛更高的抗真菌和抗菌效率，为用TNRs对植入物进行表面改性奠定了基础。
