Chen Anhui, Wang Yulong, Shao Ying, Huang Bo
Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, China; Department of Food and Biology, Xuzhou Institute of Technology, Xuzhou, China.
Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, China.
Int J Med Mushrooms. 2017;19(1):87-91. doi: 10.1615/IntJMedMushrooms.v19.i1.90.
Cordyceps militaris has been used in traditional Chinese medicine for many years, but its frequent degeneration during continuous maintenance in culture can lead to substantial commercial losses. In this study, a degenerated strain of C. militaris was obtained by subculturing a wild-type strain through 10 successive subcultures. The relative abundance of the 2 mating types seems to be out of balance in the degenerated strain. By cross-mating 4 single-ascospore isolates (2 for MAT 1-1 and 2 for MAT 1-2) from the degenerated strain, we were able to restore fruiting body production to wild-type levels. The rejuvenated strain not only produced well-developed fruiting bodies but also accumulated more cordycepin and adenosine than either the original wild-type strain or the degenerated strain. These new characteristics remained stable after 4 successive transfers, which indicates that the method used to rejuvenate the degenerated strain in this study is an effective approach.
北虫草已在传统中药中使用多年，但在连续传代培养过程中其频繁退化会导致巨大的商业损失。在本研究中，通过将野生型菌株连续传代10次获得了一株退化的北虫草菌株。在退化菌株中，两种交配型的相对丰度似乎失衡。通过对来自退化菌株的4个单孢分离株（MAT 1-1的2个和MAT 1-2的2个）进行杂交，我们能够将子实体产量恢复到野生型水平。复壮后的菌株不仅产生了发育良好的子实体，而且比原始野生型菌株或退化菌株积累了更多的虫草素和腺苷。这些新特性在连续传代4次后保持稳定，这表明本研究中用于使退化菌株复壮的方法是一种有效的方法。
