Fitzharris P, Cromwell O, Moqbel R, Hartnell A, Walsh G M, Harvey C, Kay A B
Department of Allergy and Clinical Immunology, Brompton Hospital, London, UK.
Immunology. 1987 Aug;61(4):449-55.
It has previously been shown that human neutrophils generate substantial quantities of LTB4 when stimulated with the calcium ionophore, A23187, or with unopsonized zymosan. We now report that normal human neutrophils produced substantial quantities of LTB4 (measured by radioimmunoassay and validated by RP-HPLC) when incubated with large non-phagocytosable IgG-coated beads (Sepharose 4B). LTB4 was identified in both the extra and intracellular compartments. The production of LTB4 was dependent upon the number of IgG-coated particles and the concentration of IgG bound to the beads. Release was maximal after a 15-30 min incubation time and was enhanced by prior activation of the neutrophils with the synthetic bacterial product f-met-leu-phe. Comparable LTB4 production was also observed when neutrophils were incubated with antigen (Aspergillus fumigatus)-coated beads sensitized with purified IgG obtained from the sera of patients with allergic bronchopulmonary aspergillosis. These results suggest a further mechanism by which neutrophils may be activated to produce inflammatory mediators in the tissues.
先前已经表明，当用钙离子载体A23187或未调理的酵母聚糖刺激时，人类中性粒细胞会产生大量的白三烯B4（LTB4）。我们现在报告，当与大型不可吞噬的IgG包被珠（琼脂糖4B）一起孵育时，正常人类中性粒细胞会产生大量的LTB4（通过放射免疫测定法测量并经反相高效液相色谱法验证）。在细胞外和细胞内区室中均鉴定出了LTB4。LTB4的产生取决于IgG包被颗粒的数量以及与珠子结合的IgG浓度。孵育15 - 30分钟后释放量最大，并且用合成细菌产物f - met - leu - phe预先激活中性粒细胞可增强释放。当中性粒细胞与用从变应性支气管肺曲霉病患者血清中获得的纯化IgG致敏的抗原（烟曲霉）包被珠一起孵育时，也观察到了相当的LTB4产生。这些结果提示了中性粒细胞在组织中被激活以产生炎症介质的另一种机制。
