健康驴的凝血因子、抗凝血蛋白活性及粘弹性分析

Characterisation of clotting factors, anticoagulant protein activities and viscoelastic analysis in healthy donkeys.

作者信息

Perez-Ecija A, Mendoza F J

机构信息

Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, Spain.

出版信息

Equine Vet J. 2017 Nov;49(6):734-738. doi: 10.1111/evj.12685. Epub 2017 Apr 24.

DOI:10.1111/evj.12685

PMID:28323357

Abstract

BACKGROUND

Studies have demonstrated differences in commonly measured haemostatic parameters between donkeys and horses. Whether clotting factors, anticoagulant protein activities and thromboelastography parameters also differ between species is still unknown.

OBJECTIVES

To characterise haemostatic parameters in healthy donkeys and to compare these with those in horses.

STUDY DESIGN

Cross-sectional study.

METHODS

Clotting factors (V, VII, VIII, IX, X, XI and XII), and antithrombin III, Protein C and Protein S activities were measured in 80 healthy Andalusian and crossbred donkeys and 40 healthy Andalusian crossbred horses with assays based on human deficient plasmas. Thromboelastography was performed in 34 donkeys using a coagulation and platelet function analyser.

RESULTS

Donkeys had shorter activated partial thromboplastin time (mean ± s.d. 33.4 ± 5.2 s vs. 38.8 ± 4.2 s; P<0.001) and higher Factor VII (1825 ± 206 vs. 1513 ± 174; P<0.001), IX (142 ± 41 vs. 114 ± 28; P<0.05) and XI (59.4 ± 14.0 vs. 27.2 ± 6.3; P<0.001) activities, whereas horses showed higher Factor X (130 ± 32 vs. 145 ± 23; P>0.05) and XII (96 ± 21 vs. 108 ± 15; P<0.001) activities. Antithrombin III (204 ± 26 vs. 174 ± 29; P<0.001), Protein C (33.16 ± 10.0 vs. 7.57 ± 1.70; P<0.001) and Protein S (median [interquartile range]: 7.8 [5.8-9.3] vs. 6.2 [5.2-7.0]; P<0.001) activities were higher in donkeys. Activated clot time (175 [159-189]), time to peak (6.5 [5.8-7.8]) and clot formation rate (26.9 [16.9-36.4]) in donkeys were shorter than reported values in horses.

MAIN LIMITATIONS

Haemostatic pathways could not be fully evaluated in donkeys because some tests are unavailable. Certain fibrinolytic parameters (plasmin, plasminogen, etc.) have not been characterised in donkeys and this may have affected our results.

CONCLUSIONS

The haemostatic system in donkeys differs from that in horses and extrapolation of reference values between these species is not appropriate.

摘要

背景

研究表明，驴和马在常见的止血参数方面存在差异。不同物种之间的凝血因子、抗凝血蛋白活性和血栓弹力图参数是否也存在差异尚不清楚。

目的

描述健康驴的止血参数，并与马的止血参数进行比较。

研究设计

横断面研究。

方法

采用基于人缺乏血浆的检测方法，对80头健康的安达卢西亚驴和杂交驴以及40匹健康的安达卢西亚杂交马的凝血因子（V、VII、VIII、IX、X、XI和XII）、抗凝血酶III、蛋白C和蛋白S活性进行了检测。使用凝血和血小板功能分析仪对34头驴进行了血栓弹力图检测。

结果

驴的活化部分凝血活酶时间较短（平均值±标准差，33.4±5.2秒对38.8±4.2秒；P<0.001），因子VII（1825±206对1513±174；P<0.001）、IX（142±41对114±28；P<0.05）和XI（59.4±14.0对27.2±6.3；P<0.001）活性较高，而马的因子X（130±32对145±23；P>0.05）和XII（96±21对108±15；P<0.001）活性较高。驴的抗凝血酶III（204±26对174±29；P<0.001）、蛋白C（33.16±10.0对7.57±1.70；P<0.001）和蛋白S（中位数[四分位间距]：7.8[5.8 - 9.3]对6.2[5.2 - 7.0]；P<0.001）活性较高。驴的活化凝血时间（175[159 - 189]）、达到峰值的时间（6.5[5.8 - 7.8]）和血栓形成率（26.9[16.9 - 36.4]）比报道的马的值短。