Gleńsk Michał, Czapińska Elżbieta, Woźniak Marta, Ceremuga Ireneusz, Włodarczyk Maciej, Terlecki Grzegorz, Ziółkowski Piotr, Seweryn Ewa
a Department of Pharmacognosy , Wroclaw Medical University , Wroclaw , Poland.
b Department of Medical Biochemistry , Wroclaw Medical University , Wroclaw , Poland.
Nutr Cancer. 2017 May-Jun;69(4):643-651. doi: 10.1080/01635581.2017.1295085. Epub 2017 Mar 21.
In Europe, both the fruits and flowers of Sambucus nigra L. have been used against cold, as well as laxative, diaphoretic, and diuretic remedies. There are also a number of commercially available food products that contain elderberry juice, puréed or dried elderberries. Recent comprehensive literature data on pharmacology and chemistry of Sambuci fructus have encouraged us to screen extracts with different polarities from this plant material against cancer cell lines. The cytotoxic activity of the ethyl acetate and aqueous acetone extracts from elderberries as well as detected triterpenoids on human colon adenocarcinoma cell line (LoVo) and human breast cancer cell line (MCF-7) was investigated by sulforhodamine B assay. Moreover, cell migration assay was conducted for triterpenoid fraction and pure compounds. Aqueous acetone extract possessed much lower IC value in cancer cell lines compared to ethyl acetate extract. The latter manifested high cytotoxicity against studied cell lines, suggesting that nonpolar compounds are responsible for the cytotoxic activity. Indeed, the phytochemical analysis revealed that ursolic and oleanolic acids are the main triterpenoids in the mentioned extract of which ursolic acid showed the highest activity with IC values of 10.7 µg/mL on MCF-7 and 7.7 µg/mL on LoVo cells.
在欧洲，黑接骨木的果实和花朵都被用于治疗感冒，以及作为泻药、发汗药和利尿药。也有许多市售食品含有接骨木果汁、接骨木果泥或干接骨木果。最近关于接骨木果实药理学和化学的综合文献数据促使我们用这种植物材料中不同极性的提取物对癌细胞系进行筛选。通过磺酰罗丹明B测定法研究了接骨木果乙酸乙酯提取物和水丙酮提取物以及检测到的三萜类化合物对人结肠腺癌细胞系（LoVo）和人乳腺癌细胞系（MCF-7）的细胞毒性活性。此外，对三萜类部分和纯化合物进行了细胞迁移测定。与乙酸乙酯提取物相比，水丙酮提取物在癌细胞系中的IC值要低得多。后者对所研究的细胞系表现出高细胞毒性，这表明非极性化合物是细胞毒性活性的原因。实际上，植物化学分析表明，熊果酸和齐墩果酸是上述提取物中的主要三萜类化合物，其中熊果酸活性最高，在MCF-7细胞系中的IC值为10.7μg/mL，在LoVo细胞系中的IC值为7.7μg/mL。
