Bandikari Ramesh, Poondla Vijayakumar, Obulam Vijaya Sarathi Reddy
Department of Biochemistry, Sri Venkateswara University, Tirupati, 517 502, India.
3 Biotech. 2014 Dec;4(6):655-664. doi: 10.1007/s13205-014-0239-4. Epub 2014 Jul 30.
The main objective of this study was to isolate the fungal strain for enhanced production of xylanase using different agro-residues and fruit peels by solid state fermentation and its potentiality was tested on the pretreated corn cob. Fermentation was carried out with Trichoderma koeningi isolate using untreated and pretreated corn cob supplemented with pineapple peel powder showed higher production of xylanase 2,869.8 ± 0.4 (IU/g) and extracellular protein 7.6 ± 0.2 (mg/g) of corn cob, in the latter than the former yielding 1,347.2 ± 0.7 (IU/g) and 4.9 ± 0.1 (mg/g) of corn cob, respectively, at pH 6.5 and incubation period for 96 h. In the FT-IR spectrum, the bands at 1,155, 1,252 and 1,738 cm had disappeared. This indicates the depolymerization of hemicellulose and the band at 1,053 cm shows the presence of β (1-4)-xylan in the pretreated corn cobs. The pretreated biomass hydrolysed with a xylanase concentration of 14 U and 6 h incubation showed mainly xylose and its oligosaccharides, which were quantified using HPLC. From the results we can conclude that pretreated energy-value and cheaply available agro-residues can be effectively used as substrates for the enhanced production of xylanase.
本研究的主要目的是通过固态发酵，利用不同的农业废弃物和果皮分离出用于提高木聚糖酶产量的真菌菌株，并在预处理的玉米芯上测试其潜力。使用科氏木霉分离株进行发酵，未处理和预处理的玉米芯添加菠萝皮粉后，在pH 6.5和培养96小时的条件下，后者玉米芯的木聚糖酶产量更高，为2,869.8 ± 0.4（IU/g），细胞外蛋白为7.6 ± 0.2（mg/g），而前者分别为1,347.2 ± 0.7（IU/g）和4.9 ± 0.1（mg/g）。在傅里叶变换红外光谱中，1,155、1,252和1,738 cm处的谱带消失。这表明半纤维素发生了解聚，1,053 cm处的谱带表明预处理的玉米芯中存在β(1-4)-木聚糖。用浓度为14 U的木聚糖酶水解预处理的生物质并孵育6小时，主要产物为木糖及其寡糖，使用高效液相色谱对其进行定量。从结果可以得出结论，预处理后的具有能量价值且廉价可得的农业废弃物可有效用作提高木聚糖酶产量的底物。
