Tanase Corneliu, Berta Lavinia, Coman Năstaca Alina, Roșca Ioana, Man Adrian, Toma Felicia, Mocan Andrei, Jakab-Farkas László, Biró Domokos, Mare Anca
Department of Pharmaceutical Botany, "George Emil Palade" University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, Târgu Mureș, 540139 Mureș, Romania.
Department of General and Inorganic Chemistry, "George Emil Palade" University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureș, 38 Gheorghe Marinescu Street, Târgu Mureș, 540139 Mureș, Romania.
Antioxidants (Basel). 2019 Oct 8;8(10):459. doi: 10.3390/antiox8100459.
Green synthesis is one of the rapid and best ways for silver nanoparticles (AgNP) synthesis. In the present study, synthesis and bioactivity of AgNPs has been demonstrated using water beech ( L.) bark extract. The physical and chemical factors such as time, metal ion solution, and pH, which play a vital role in the AgNPs synthesis, were assessed. The AgNPs were characterized by ultraviolet-visible (UV-Vis) spectrometry, Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM). Antioxidant and antimicrobial activity of the obtained AgNPs was evaluated. AgNPs were characterized by color change pattern, and the broad peak obtained at 420-475 nm with UV-Vis confirmed the synthesis of AgNPs. FT-IR results confirmed that phenols and proteins of beech bark extract are mainly responsible for capping and stabilization of synthesized AgNPs. TEM micrographs showed spherical or rarely polygonal and triangular particles with an average size of 32 nm at pH = 9, and 62 nm at pH = 4. Furthermore, synthesized AgNPs were found to exhibit antioxidant activity and have antibacterial effect against methicillin-resistant (MRSA), , and . These results indicate that bark extract of L. is suitable for synthesizing stable AgNPs, which act as an excellent antimicrobial agent.
绿色合成是银纳米颗粒（AgNP）合成的快速且最佳方法之一。在本研究中，已证明使用水青冈（L.）树皮提取物合成AgNPs并具有生物活性。评估了时间、金属离子溶液和pH值等在AgNPs合成中起关键作用的物理和化学因素。通过紫外可见（UV-Vis）光谱、傅里叶变换红外光谱（FT-IR）和透射电子显微镜（TEM）对AgNPs进行了表征。评估了所得AgNPs的抗氧化和抗菌活性。通过颜色变化模式对AgNPs进行了表征，UV-Vis在420 - 475 nm处获得的宽峰证实了AgNPs的合成。FT-IR结果证实，水青冈树皮提取物中的酚类和蛋白质主要负责合成的AgNPs的封端和稳定。TEM显微照片显示，在pH = 9时为球形，很少为多边形和三角形颗粒，平均尺寸为32 nm，在pH = 4时为62 nm。此外，发现合成的AgNPs具有抗氧化活性，并且对耐甲氧西林金黄色葡萄球菌（MRSA）、[此处原文缺失两种细菌名称]具有抗菌作用。这些结果表明，水青冈L.的树皮提取物适合合成稳定的AgNPs，其可作为一种优异的抗菌剂。
