Keshri V, Singh Dhananjaya P, Prabha R, Rai A, Sharma A K
National Bureau of Agriculturally Important Microorganisms, Indian Council of Agricultural Research, Kushmaur, Maunath Bhanjan, 275101, UP, India.
Indian Agricultural Statistical Research Institute, Indian Council of Agricultural Research, Library Avenue, Pusa, New Delhi, 110012, India.
3 Biotech. 2014 Feb;4(1):91-95. doi: 10.1007/s13205-013-0131-7. Epub 2013 Apr 4.
In pathogenic bacteria, identification of essential proteins which are non-homologous to the host plants represents potential antimicrobial targets. We applied subtractive genomics approach for the identification of novel antimicrobial targets in Xanthomonas oryzae pv. oryzae PXO99A, the causative agent of bacterial blight in rice. Comparative analysis was performed through BLAST available with the NCBI. The analysis revealed that 27 essential protein sequences out of 4,988 sequences of X. oryzae pv. oryzae PXO99A are non-homologous to Oryza sativa. Subsequent analysis of 27 essential proteins revealed their involvement in different metabolic activities such as transport activity, DNA binding, structural constituent of ribosome, cell division, translation, and plasma membrane. These 27 proteins were analyzed for virulence and novelty and out of 27, three essential non-homologous proteins were found to be the novel antimicrobial targets.
在致病细菌中，鉴定与宿主植物无同源性的必需蛋白代表了潜在的抗菌靶点。我们应用消减基因组学方法来鉴定水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae）PXO99A中的新型抗菌靶点，该病菌是水稻白叶枯病的病原体。通过美国国立医学图书馆（NCBI）提供的BLAST进行了比较分析。分析表明，水稻白叶枯病菌PXO99A的4988个序列中的27个必需蛋白序列与水稻（Oryza sativa）无同源性。随后对这27种必需蛋白的分析揭示了它们参与不同的代谢活动，如转运活性、DNA结合、核糖体的结构组成、细胞分裂、翻译和质膜。对这27种蛋白质进行了毒力和新颖性分析，在这27种蛋白质中，发现有三种必需的非同源蛋白质是新型抗菌靶点。
