Ehrampoush Mohammad H, Sadeghi Abbas, Ghaneian Mohammad T, Bonyadi Ziaeddin
Department of Environmental Health Engineering, Environmental Science and Technology Research Center, Shahid Sadoughi University of Medical Science, Yazd, Iran.
Department of Environmental Health, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran.
AMB Express. 2017 Dec;7(1):68. doi: 10.1186/s13568-017-0366-5. Epub 2017 Mar 21.
Diazinon is an organophosphate compound that inhibits the activity of acetylcholinesterase. Standards of the World Health Organization and Environmental Protection Agency for diazinon concentration in water are 0.1 and 9 × 10 mg/L, respectively. The aim of this study was the optimization of diazinon biodegradation from aqueous solutions by Saccharomyces cerevisiae using the response surface methodology (RSM). Harvested cells of S. cerevisiae were locally purchased from the Iranian Research Organization for Science and Technology. To obtain the optimum condition for diazinon biodegradation using RSM, input parameters included the initial concentration of diazinon (0.01-10 mg/L), concentration of S. cerevisiae (0.5-5%), pH (4-10), and retention time (1-30 h). The research study had a central composite design where one of the methods was RSM. According to the results, the observed values of the removal efficiency of diazinon were variable in the range of 23-96. The highest removal rate was obtained as 96% under the initial diazinon concentration of 2.5 mg/L, S. cerevisiae concentration of 3.88%, pH of 5.5, and retention time of 22.75 h. The results displayed that the removal efficiency of diazinon had a direct relationship with the concentration of S. cerevisiae and retention time, and an inverse relationship with pH and the initial concentration of diazinon. We can conclude that S. cerevisiae has the ability to remove diazinon with the lowest cost and a high efficiency.
二嗪农是一种抑制乙酰胆碱酯酶活性的有机磷化合物。世界卫生组织和环境保护局规定的水中二嗪农浓度标准分别为0.1和9×10毫克/升。本研究的目的是采用响应面法（RSM）优化酿酒酵母对水溶液中二嗪农的生物降解。酿酒酵母的收获细胞是从伊朗科学技术研究组织当地购买的。为了使用RSM获得二嗪农生物降解的最佳条件，输入参数包括二嗪农的初始浓度（0.01 - 10毫克/升）、酿酒酵母的浓度（0.5 - 5%）、pH值（4 - 10）和保留时间（1 - 30小时）。该研究采用中心复合设计，其中一种方法是RSM。根据结果，二嗪农去除效率的观测值在23% - 96%范围内变化。在二嗪农初始浓度为2.5毫克/升、酿酒酵母浓度为3.88%、pH值为5.5和保留时间为22.75小时的条件下，获得了最高去除率96%。结果表明，二嗪农的去除效率与酿酒酵母的浓度和保留时间呈正相关，与pH值和二嗪农的初始浓度呈负相关。我们可以得出结论，酿酒酵母有能力以低成本和高效率去除二嗪农。
