Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, DHHS, Bethesda, MD, USA.
Oral Dis. 2017 Sep;23(6):801-808. doi: 10.1111/odi.12667. Epub 2017 Apr 27.
The goals of this study were to (i) establish a useful miniature pig (minipig) model for irradiation-induced oral mucositis and (ii) evaluate the effect of Tempol to prevent its development.
Minipigs were irradiated with 6 Gy for five consecutive days targeting the entire oral cavity. To prevent radiation damage, minipigs were treated with 30 mg kg Tempol 10 min before irradiation (n = 4), while the radiation-alone group was similarly injected with saline (n = 4). Lesions were graded using an oral mucositis score and visual inspection every 3 days, and biopsy of multiple sites was performed at day 18. Weight and chest and abdominal circumferences were measured every 3 days.
Lesions began about 12 days after the first irradiation fraction and healed about 30 days after irradiation. Epithelial thickness was calculated on the lingual and buccal mucosa on the 18th day after the first irradiation fraction. Tempol provided modest protection from ulceration after irradiation using this treatment strategy.
This study established a useful large animal model for irradiation-induced oral mucositis and showed modest beneficial effects of Tempol in limiting tissue damage. The latter finding may be potentially valuable in preventing oral mucositis in patients receiving irradiation for head and neck cancers.
本研究的目的是（i）建立一种有用的小型猪（迷你猪）放射性口腔黏膜炎模型，（ii）评估 Tempol 预防其发展的效果。
将迷你猪连续 5 天用 6 Gy 照射整个口腔。为了防止辐射损伤，在照射前 10 分钟用 30mg/kg Tempol 治疗迷你猪（n=4），而单纯照射组则用生理盐水类似注射（n=4）。使用口腔黏膜炎评分和每天 3 次的肉眼观察来分级病变，并在第 18 天进行多个部位的活检。每 3 天测量体重和胸部及腹部周长。
病变在第一次照射后约 12 天开始，并在照射后约 30 天愈合。在第一次照射后第 18 天计算舌和颊黏膜的上皮厚度。使用这种治疗策略，Tempol 对放射性溃疡提供了适度的保护。
本研究建立了一种有用的大型动物放射性口腔黏膜炎模型，并显示 Tempol 对限制组织损伤有适度的有益作用。后者的发现可能对预防头颈部癌症接受放疗的患者的口腔黏膜炎具有潜在价值。
