Mourembou Gaël, Rathored Jaishriram, Lekana-Douki Jean Bernard, Ndjoyi-Mbiguino Angélique, Khelaifia Saber, Robert Catherine, Armstrong Nicholas, Raoult Didier, Fournier Pierre-Edouard
URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France.
Ecole Doctorale Régionale d'Afrique Centrale, B.P. 876, Franceville, Gabon.
Curr Microbiol. 2016 Dec;73(6):867-877. doi: 10.1007/s00284-016-1137-2. Epub 2016 Sep 15.
The identification of human-associated bacteria is very important to control infectious diseases. In recent years, we diversified culture conditions in a strategy named culturomics, and isolated more than 100 new bacterial species and/or genera. Using this strategy, strain GM7, a strictly anaerobic gram-negative bacterium was recently isolated from a stool specimen of a healthy Gabonese patient. It is a motile coccobacillus without catalase and oxidase activities. The genome of Gabonibacter massiliensis is 3,397,022 bp long with 2880 ORFs and a G+C content of 42.09 %. Of the predicted genes, 2,819 are protein-coding genes, and 61 are RNAs. Strain GM7 differs from the closest genera within the family Porphyromonadaceae both genotypically and in shape and motility. Thus, we propose that strain GM7 (=CSUR P2336 = DSM 101039) is the type strain of the new genus Gabonibacter gen. nov. and the new species G. massiliensis gen. nov., sp. nov.
鉴定与人类相关的细菌对于控制传染病非常重要。近年来，我们采用一种名为“ culturomics ”的策略使培养条件多样化，并分离出100多种新的细菌物种和/或属。利用这一策略，最近从一名健康加蓬患者的粪便标本中分离出菌株GM7，这是一种严格厌氧的革兰氏阴性菌。它是一种运动性的球杆菌，无过氧化氢酶和氧化酶活性。马西利亚加蓬杆菌的基因组长度为3397022 bp，有2880个开放阅读框，G+C含量为42.09%。在预测的基因中，2819个是蛋白质编码基因，61个是RNA。菌株GM7在基因型、形状和运动性方面与卟啉单胞菌科中最接近的属不同。因此，我们提议菌株GM7（=CSUR P2336 = DSM 101039）是新属加蓬杆菌属（Gabonibacter gen. nov.）和新物种马西利亚加蓬杆菌（G. massiliensis gen. nov., sp. nov.）的模式菌株。
