Benemérita Universidad Autónoma de Puebla, Facultad de Estomatología, Academia de Odontología Pediátrica, Puebla, México.
Universidad Autónoma del Estado de México, Facultad de Odontología, Centro de Investigación y Estudios Avanzados en Odontología (CIEAO), Toluca, Estado de México, Mexico.
Microsc Res Tech. 2021 Aug;84(8):1628-1637. doi: 10.1002/jemt.23723. Epub 2021 Feb 12.
Reinforced glass ionomer cements have been widely used in pediatric dentistry to prevent dental caries. However, the influence of biomaterial light-curing and its anti-cariogenic effects remain unclear. This study evaluates the influence of the light-curing time on fluoride release, surface topography, and bacterial adhesion in two types of resin-modified glass ionomer cements (RMGICs). One hundred disks were made, and samples were divided into two groups (n = 50 per group), according to each dental material (Vitremer™ and Ketac™ N100), and also divided into different light-cured times (10, 20, 30, 40, and 60 s). They were placed in phosphate-buffered saline solution (PBS) to measure the fluoride release. Subsequently, an independent sample of RMGICs per group was examined using atomic force microscopy (AFM). Four disks per group were incubated in a brain heart infusion (BHI) medium that was inoculated with Streptococcus mutans GS5 to evaluate the bacterial adhesion by 3-4, [5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay). The fluoride release was related to the light-curing time and gradually decreased as the light-curing time increased in both materials. Surface topography in Vitremer™ presents more irregular surfaces than Ketac™ N100. For S. mutans adhesion, the smallest number of cells per milliliter (cell/ml) was found at 40 s for Vitremer™ and at 30 s for Ketac™ N100. Thus, the shorter light-curing times allowed for major fluoride release in both materials. However, the RMGICs showed different patterns of bacterial adhesion according to the brand and light-curing time.
玻璃离子水门汀在儿童牙科中被广泛用于预防龋齿。然而，生物材料的光固化及其抗龋效果的影响尚不清楚。本研究评估了光固化时间对两种树脂改性玻璃离子水门汀（RMGIC）的氟释放、表面形貌和细菌黏附的影响。制作了 100 个圆盘，根据每种牙科材料（VitremerTM 和 KetacTM N100）将样品分为两组（每组 50 个样本），并进一步分为不同的光固化时间（10、20、30、40 和 60 s）。将它们置于磷酸盐缓冲盐水溶液（PBS）中以测量氟释放。随后，每组 RMGIC 的独立样本使用原子力显微镜（AFM）进行检查。每组 4 个圆盘在接种了变形链球菌 GS5 的脑心浸液（BHI）培养基中孵育，通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物细胞活力测定法（MTT 测定法）评估细菌黏附。氟释放与光固化时间有关，并且在两种材料中随着光固化时间的增加而逐渐减少。VitremerTM 的表面形貌比 Ketac™ N100 呈现更不规则的表面。对于 S. mutans 黏附，在 Vitremer™ 中发现每毫升（cell/ml）最小的细胞数为 40 s，在 Ketac™ N100 中为 30 s。因此，两种材料中较短的光固化时间允许主要的氟释放。然而，RMGIC 根据品牌和光固化时间表现出不同的细菌黏附模式。
