Department of Pharmacy, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Department of nephrology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Sci Rep. 2017 Mar 22;7:45111. doi: 10.1038/srep45111.
Danggui Buxue Tang (DBT) is a classic Chinese herbal formula which consists of Astragali mongholici Radix and Angelica sinensis Radix (ASR). For chemical ingredients, HPLC were performed. Results showed compared with single herbs, DBT decoction could promote the dissolution of ingredients such as ferulic acid and calycosin. Furthermore, when ratio of AMR to ASR was 5 to 1, synthetic score was the best. For angiogenesis, normal and injured zebrafish model were applied. Results showed DBT and its ingredients had angiogenesis effects on Sub Intestinal vessels (SIVs) of normal zebrafish. Meanwhile, DBT and its single herbs could also recover Inter-Segmental Vessels (ISVs) injured by VRI. Angiogenesis effects of DBT on ISVs were better than single herbs. AMR extract, Total Saponins of AMR, Polysaccharide of ASR, ferulic acid, calycosin and calycosin-7-glucoside could be effective ingredients for angiogenisis. For endothelium functions, Lysoph-Osphatidyl choline was used to damage rat endothelial function of thoracic aorta. The results showed DBT and its single herbs could improve endothelial dysfunctions in dose-dependence. Both ferulic acid and calycosin-7-glucoside could also improve endothelium dysfunction in dose dependence. Therefore, compatibility of DBT was reasonable. Compared with single herbs, DBT could promote dissolution of effective ingredients, enhance angiogenesis and relieve endothelial dysfunction.
当归补血汤（DBT）是一种经典的中草药配方，由黄芪和当归组成。对于化学成分，采用高效液相色谱法（HPLC）进行分析。结果表明，与单味药相比，DBT 汤可以促进阿魏酸和毛蕊异黄酮苷等成分的溶解。此外，当 AMR 与 ASR 的比例为 5:1 时，合成评分最佳。对于血管生成，应用正常和受伤的斑马鱼模型。结果表明，DBT 及其成分对正常斑马鱼的肠下血管（SIVs）具有血管生成作用。同时，DBT 及其单味药也可以恢复由 VRI 损伤的节间血管（ISVs）。DBT 对 ISVs 的血管生成作用优于单味药。AMR 提取物、AMR 总皂苷、ASR 多糖、阿魏酸、毛蕊异黄酮苷和毛蕊异黄酮-7-葡萄糖苷可以作为有效的血管生成成分。对于血管内皮功能，溶血磷脂酰胆碱用于损伤大鼠胸主动脉内皮功能。结果表明，DBT 及其单味药可以剂量依赖性地改善内皮功能障碍。阿魏酸和毛蕊异黄酮-7-葡萄糖苷也可以剂量依赖性地改善内皮功能障碍。因此，DBT 的配伍是合理的。与单味药相比，DBT 可以促进有效成分的溶解，增强血管生成，缓解内皮功能障碍。
