Gomez-Cabrera Mari Carmen, Garcia-Valles Rebeca, Rodriguez-Mañas Leocadio, Garcia-Garcia Francisco Jose, Olaso-Gonzalez Gloria, Salvador-Pascual Andrea, Tarazona-Santabalbina Francisco Jose, Viña Jose
Department of Physiology, University of Valencia, Fundación Investigación Hospital Clínico Universitario/INCLIVA, Spain.
Servicio de Geriatría, Hospital Universitario de Getafe, Ministerio de Sanidad y Consumo, Madrid, Spain. Red Temática de Investigación Cooperativa en Envejecimiento y Fragilidad (RETICEF), Instituto de Salud Carlos III.
J Gerontol A Biol Sci Med Sci. 2017 Jul 1;72(7):885-891. doi: 10.1093/gerona/glw337.
The development of animal models to study human frailty is important to test interventions to be translated to the clinical practice. The aim of this work was to develop a score for frailty in experimental animals based in the human frailty phenotype. We also tested the effect of physical inactivity in the development of frailty as determined by our score. Male C57Bl/6J mice, individually caged, were randomly assigned to one of two groups: sedentary (inactive) or spontaneous wheel-runners. We compared the sedentary versus the active lifestyle in terms of frailty by evaluating the clinical criteria used in humans: unintentional weight loss; poor endurance (running time); slowness (running speed); weakness (grip strength), and low activity level (motor coordination) at five different ages: 17, 20, 23, 26 and 28 months of age. Each criterion had a designated cut-off point to identify the mice with the lowest performance. Lifelong spontaneous exercise significantly retards frailty. On the contrary sedentary animals become frail as they age. Thus, physical inactivity is a model of frailty in experimental animals. Our frailty score provides a tool to evaluate interventions in mice prior to translating them to clinical practice.
开发用于研究人类衰弱的动物模型对于测试可转化为临床实践的干预措施非常重要。这项工作的目的是基于人类衰弱表型开发一种实验动物衰弱评分。我们还测试了不运动对由我们的评分所确定的衰弱发展的影响。单独饲养的雄性C57Bl/6J小鼠被随机分为两组：久坐不动（不活动）组或自发使用转轮跑步组。我们通过评估人类使用的临床标准，比较了久坐不动与积极生活方式在衰弱方面的差异：非故意体重减轻；耐力差（跑步时间）；行动迟缓（跑步速度）；虚弱（握力），以及在五个不同年龄（17、20、23、26和28月龄）时的低活动水平（运动协调性）。每个标准都有一个指定的临界点来识别表现最差的小鼠。终身自发运动显著延缓衰弱。相反，久坐不动的动物随着年龄增长会变得衰弱。因此，不运动是实验动物衰弱的一种模型。我们的衰弱评分提供了一种工具，可在将干预措施转化为临床实践之前评估小鼠中的干预措施。
