Hussain S K Asraf, Srivastava Anima, Tyagi Ashish, Shandilya Umesh Kumar, Kumar Ashwani, Kumar Sachin, Panwar Surbhi, Tyagi Amrish Kumar
Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal, 132001, Haryana, India.
Seth Jai Parkash Mukand Lal Institute of Engineering and Technology (JMIT), Radaur, 135133, Haryana, India.
3 Biotech. 2016 Jun;6(1):90. doi: 10.1007/s13205-016-0401-2. Epub 2016 Mar 10.
Conjugated Linoleic Acid (CLA), a fatty acid with high nutraceutical value is produced in rumen by resident bacterial species, especially Butyrivibrio spp. The present study was undertaken to examine the diversity of indigenous Butyrivibrio spp. from rumen liquor of Indian ruminants. The isolates were screened for their CLA production capability at different level of linoleic acid (LA) (0, 200, 400, 600, 800 μg/ml) at different time intervals (0, 2, 4, 6, 12, and 24 h). A total of more than 300 anaerobic cultures were isolated and 31 of them were identified as Butyrivibrio spp. based on morphological, biochemical and molecular characterization. Further, molecular characterization revealed that a large portion (67.7 %) of isolated Butyrivibrio belonged to Butyrivibrio fibrisolvens (B. fibrisolvens) species which is considered to be the most active bacteria amongst the rumen bacteria populace in terms of CLA production. Bacterial isolate VIII (strain 4a) showed highest CLA production ability (140.77 μg/ml) when incubated at 200 μg/ml LA for 2 h, which is 240 % higher than the isolate XXVII, Butyrivibrio proteoclasticus (B. proteoclasticus) showing lowest CLA production (57.28 μg/ml) amongst the screened isolates. It was evident from the observations recorded during the course of experiments that CLA production ability is strain specific and thus did not follow a single pattern. CLA production also varied with time of incubation and concentration of free linoleic acid supplemented in the growth medium. The results of these findings put forward a strain that is high CLA producer and can be further exploited as an additive for enhancing meat and milk quality in ruminants.
共轭亚油酸（CLA）是一种具有高营养价值的脂肪酸，由瘤胃中的常驻细菌物种产生，特别是丁酸弧菌属。本研究旨在检测印度反刍动物瘤胃液中本土丁酸弧菌属的多样性。在不同时间间隔（0、2、4、6、12和24小时），在不同亚油酸（LA）水平（0、200、400、600、800μg/ml）下筛选分离株的CLA生产能力。共分离出300多个厌氧培养物，其中31个根据形态、生化和分子特征被鉴定为丁酸弧菌属。此外，分子特征显示，分离出的丁酸弧菌中很大一部分（67.7%）属于溶纤维丁酸弧菌（B. fibrisolvens）物种，该物种被认为是瘤胃细菌群体中CLA生产方面最活跃的细菌。细菌分离株VIII（菌株4a）在200μg/ml LA下孵育2小时时显示出最高的CLA生产能力（140.77μg/ml），比筛选分离株中CLA产量最低的解蛋白丁酸弧菌（B. proteoclasticus）分离株XXVII高240%（57.28μg/ml）。从实验过程中记录的观察结果可以明显看出，CLA生产能力具有菌株特异性，因此不遵循单一模式。CLA产量也随孵育时间和生长培养基中添加的游离亚油酸浓度而变化。这些研究结果提出了一种CLA高产菌株，可进一步用作添加剂，以提高反刍动物的肉和奶品质。
