Rao Sunita, Shekhawat Gyan Singh
Department of Science, Biyani Girls College, Jaipur, Rajasthan, 302023, India.
Department of Botany, Jai Narain Vyas University, Jodhpur, Rajasthan, 342008, India.
3 Biotech. 2016 Dec;6(2):244. doi: 10.1007/s13205-016-0550-3. Epub 2016 Nov 15.
This study elaborates the consequences of oxidative stress caused by copper oxide (CuO) and titanium dioxide (TiO) nanoparticles (NPs) in Brassica juncea. Effect of these two NPs on plant physiology, reactive oxygen scavenging enzyme system (ascorbate peroxidase, catalase, superoxide dismutase), proline content and lipid peroxidation has been estimated in leaves as well as root tissues. Bioaccumulation of NPs has also been evaluated in the current study and the interrelated cascade of the enzymatic system with HO production was identified. The uptake of NPs in plant leaves was confirmed by scanning electron microscopy, X-ray diffraction, and Fourier Transform Infrared Spectroscopy. Plant growth was found to be diminished with elevated levels of CuO NPs whereas TiO NPs had shown an opposite effect. The plant species accumulated lower concentration of NPs and displayed considerable tolerance against stress, probably due to well-organized and coordinated defense system at the root and shoot level by the intonation of antioxidative enzymes.
本研究阐述了氧化铜（CuO）和二氧化钛（TiO₂）纳米颗粒（NPs）在芥菜中引起的氧化应激后果。已评估了这两种纳米颗粒对植物生理学、活性氧清除酶系统（抗坏血酸过氧化物酶、过氧化氢酶、超氧化物歧化酶）、脯氨酸含量和脂质过氧化在叶片及根组织中的影响。本研究还评估了纳米颗粒的生物累积情况，并确定了酶系统与H₂O₂产生的相关级联反应。通过扫描电子显微镜、X射线衍射和傅里叶变换红外光谱证实了植物叶片对纳米颗粒的吸收。发现随着CuO纳米颗粒水平的升高，植物生长受到抑制，而TiO₂纳米颗粒则表现出相反的效果。该植物物种积累了较低浓度的纳米颗粒，并表现出相当强的抗逆性，这可能是由于通过上调抗氧化酶在根和地上部分形成了组织良好且协调的防御系统。
