Department of Preventive Dentisty, The Research Center of Stomatology, Stomatology Hospital, Xi'an Jiaotong University, No. 98 West-Five Road, Xi'an 710004, China; Department of Stomatology, Yulin First Hospital, No. 93 Yuxi Avenue, Yulin, 719000 Shaanxi Province, China.
Department of Stomatology, Yulin First Hospital, No. 93 Yuxi Avenue, Yulin, 719000 Shaanxi Province, China.
Int J Biol Macromol. 2017 Aug;101:341-347. doi: 10.1016/j.ijbiomac.2017.03.098. Epub 2017 Mar 19.
Repair of craniofacial bony defects remains a challenge for surgeons due to the delicate and complex anatomy of the craniofacial skeleton. Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy. In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels. The SDF-1α encapsulated in CS/CMCS NPs within CS/GP hydrogels showed significantly sustained release effect. The cumulative release of SDF-1α was only 40% during 28d. The data from rat calvarial defects model revealed that the SDF-1α/CS/CMCS NPs embedded hydrogels group could significantly promote the new bone formation (38.5±4.5%), compared to that of the SDF-1α embedded hydrogels group (26.3±7.25%, p<0.05) and the control group (8.64±4.8%, p<0.01). Histological data also confirmed this difference. This study demonstrated the potential applications of nanoparticulate injectable hydrogels for sustained release SDF-1α on bone tissue regeneration.
颅颌面骨缺损的修复仍然是外科医生面临的挑战，这是由于颅颌面骨骼的精细和复杂的解剖结构。基质细胞衍生因子-1α（SDF-1α）是一种重要的趋化因子，在间充质干细胞（MSC）归巢中起着关键作用，然而，半衰期短和容易被酶降解等缺点使其疗效相对较低。在这项工作中，制备了 SDF-1α/壳聚糖/羧甲基壳聚糖纳米颗粒（SDF-1α/CS/CMCS NPs），并对其进行了各种参数的表征，包括形态、粒径、Zeta 电位、载药效率和从温敏壳聚糖/β-甘油磷酸二钠（CS/GP）水凝胶中的释放特性。包封在 CS/CMCS NPs 中的 SDF-1α 在 CS/GP 水凝胶中表现出明显的持续释放效果。在 28d 内，SDF-1α 的累积释放仅为 40%。大鼠颅骨缺损模型的数据显示，SDF-1α/CS/CMCS NPs 嵌入水凝胶组可显著促进新骨形成（38.5±4.5%），与 SDF-1α 嵌入水凝胶组（26.3±7.25%，p<0.05）和对照组（8.64±4.8%，p<0.01）相比。组织学数据也证实了这一差异。这项研究表明，纳米颗粒可注射水凝胶在骨组织再生中具有作为 SDF-1α 持续释放载体的潜力。
