Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yet-Sen University, Guangzhou, People's Republic of China.
Int J Nanomedicine. 2020 Nov 24;15:9337-9353. doi: 10.2147/IJN.S270681. eCollection 2020.
Autologous bone grafts are the gold standard for treating bone defects. However, limited bone supply and morbidity at the donor site restrict its extensive use. Therefore, developing bone graft materials as an alternative to autologous grafts has gained considerable attention. Injectable hydrogels endowed with osteogenic potential have the ability to fill irregular bone defects using minimally invasive procedures and have thus been attracting researchers' attention. However, from a clinical perspective, most fabrication methods employed for the current injectable osteogenic hydrogels are difficult and inconvenient. In the current study, we fabricated an injectable osteogenic hydrogel using a simple and convenient strategy.
Gelatin-methacryloyl (GelMA) pre-polymer was synthetized. Nano silicate (SN) and stromal cell-derived factor-1 alpha (SDF-1α) were introduced into the pre-polymer to achieve injectability, controlled release property, excellent osteogenic ability, and efficient stem cell homing.
The GelMA-SN-SDF-1α demonstrated excellent injectability via a 17-G needle at room temperature. The loaded SDF-1α exhibited a long-term controlled release pattern and efficiently stimulated MSC migration and homing. The GelMA-SN-SDF-1α hydrogel amplified cell spreading, migration, osteogenic-related biomarker expression, and matrix mineralization. The GelMA-SN-SDF-1α hydrogel filled critical-sized calvaria defects in rats and demonstrated excellent bone regeneration ability, as assessed using micro-CT scanning and histomorphometric staining.
The GelMA-SN-SDF-1α hydrogel provides a simple and convenient strategy for the fabrication of injectable osteogenic graft materials.
自体骨移植物是治疗骨缺损的金标准。然而，有限的骨供应和供体部位的发病率限制了其广泛应用。因此，开发骨移植物材料作为自体移植物的替代品引起了相当大的关注。具有成骨潜力的可注射水凝胶能够通过微创程序填充不规则的骨缺损，因此引起了研究人员的关注。然而，从临床角度来看，目前用于可注射成骨水凝胶的大多数制造方法都很困难和不方便。在本研究中，我们采用简单方便的策略制备了可注射成骨水凝胶。
合成了明胶-甲基丙烯酰（GelMA）预聚物。将纳米硅酸盐（SN）和基质细胞衍生因子-1 ɑ（SDF-1ɑ）引入预聚物中，以实现可注射性、控制释放性能、优异的成骨能力和高效的干细胞归巢。
室温下通过 17-G 针头，GelMA-SN-SDF-1α 表现出优异的可注射性。负载的 SDF-1ɑ 呈现出长期的控制释放模式，并能有效刺激 MSC 迁移和归巢。GelMA-SN-SDF-1α 水凝胶扩增细胞铺展、迁移、成骨相关生物标志物表达和基质矿化。GelMA-SN-SDF-1α 水凝胶填充大鼠临界颅骨缺损，并通过 micro-CT 扫描和组织形态计量学染色评估，显示出优异的骨再生能力。
GelMA-SN-SDF-1α 水凝胶为可注射成骨移植物材料的制备提供了一种简单方便的策略。
