Assis Renata Pires, Arcaro Carlos Alberto, Gutierres Vânia Ortega, Oliveira Juliana Oriel, Costa Paulo Inácio, Baviera Amanda Martins, Brunetti Iguatemy Lourenço
Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, São Paulo 14800-903, Brazil.
Int J Mol Sci. 2017 Mar 23;18(4):332. doi: 10.3390/ijms18040332.
Combination therapy using natural antioxidants to manage diabetes mellitus and its complications is an emerging trend. The aim of this study was to investigate the changes promoted by treatment of streptozotocin (STZ)-diabetic rats with yoghurt enriched with the bioactives curcumin, lycopene, or bixin (the latter two being carotenoids). Antioxidants were administered individually, or as mixtures, and biomarkers of metabolic and oxidative disturbances, particularly those associated with cardiovascular risk, were assessed. Treatment of STZ-diabetic rats with natural products individually decreased glycemia, triacylglycerol, total-cholesterol, oxidative stress biomarkers, including oxidized low-density lipoprotein (ox-LDL), and increased the activities of antioxidant enzymes. Individual carotenoids increased both high-density lipoprotein (HDL) and paraoxonase levels, whereas curcumin increased only paraoxonase. Treatments with mixtures of curcumin and lycopene or bixin had combined effects, decreasing biomarkers of carbohydrate and lipid disturbances (curcumin effect), increasing the HDL levels (carotenoids effects) and mitigating oxidative stress (curcumin and carotenoids effects). The combined effects also led to prevention of the LDL oxidation, thereby mitigating the cardiovascular risk in diabetes. These findings provide evidence for the beneficial effect of curcumin and carotenoid mixtures as a supplementation having antioxidant and antiatherogenic potentials, thus appearing as an interesting strategy to be studied as a complementary therapy for diabetic complications.
使用天然抗氧化剂联合治疗糖尿病及其并发症是一种新趋势。本研究的目的是调查用富含生物活性成分姜黄素、番茄红素或胭脂树橙（后两者为类胡萝卜素）的酸奶治疗链脲佐菌素（STZ）诱导的糖尿病大鼠所带来的变化。抗氧化剂单独给药或混合给药，并评估代谢和氧化紊乱的生物标志物，特别是与心血管风险相关的标志物。用天然产物单独治疗STZ诱导的糖尿病大鼠可降低血糖、三酰甘油、总胆固醇、氧化应激生物标志物，包括氧化型低密度脂蛋白（ox-LDL），并提高抗氧化酶的活性。单独的类胡萝卜素可提高高密度脂蛋白（HDL）和对氧磷酶水平，而姜黄素仅提高对氧磷酶水平。用姜黄素与番茄红素或胭脂树橙的混合物治疗具有联合效应，可降低碳水化合物和脂质紊乱的生物标志物（姜黄素效应），提高HDL水平（类胡萝卜素效应）并减轻氧化应激（姜黄素和类胡萝卜素效应）。联合效应还可预防LDL氧化，从而减轻糖尿病患者的心血管风险。这些发现为姜黄素和类胡萝卜素混合物作为具有抗氧化和抗动脉粥样硬化潜力的补充剂的有益作用提供了证据，因此似乎是一种值得研究的作为糖尿病并发症辅助治疗的有趣策略。
