Uen W C, Luka J, Pearson G R
Department of Microbiology, Georgetown University Medical Center, Washington, DC 20007.
Int J Cancer. 1988 Apr 15;41(4):479-82. doi: 10.1002/ijc.2910410402.
A 3-step enzyme-linked immunosorbent assay (ELISA) was developed for detecting IgA antibodies to purified Epstein-Barr virus (EBV) polypeptides. The 3-step procedure included the use of a mouse anti-human IgA monoclonal antibody (MAb) to amplify the IgA reaction. The 2 major EBV proteins used in this assay were the 125-kDa component (gp125) associated with the viral capsid antigen (VCA) complex and a major glycoprotein (gp250/200) associated with the membrane antigen (MA) complex. Eighty-two sera were tested on ELISA plates containing either both of the glycoproteins or each one separately. These included 45 IgA antibody-positive sera from patients with nasopharyngeal carcinoma (NPC). With these sera, there was a good correlation, both qualitatively and quantitatively, between results with the immunofluorescence (IF) and ELISA procedures. Although most IgA antibody-positive sera contained antibodies reactive with both gp125 and gp250/200, a number of sera contained antibodies reactive with one of the glycoproteins but not with both. The data indicated that both of these glycoproteins should be used in assays for detecting IgA antibodies to EBV, to avoid false-negative results. This assay should be useful for screening large populations for IgA antibodies to EBV and also possibly for monitoring disease course in patients with NPC.
开发了一种三步酶联免疫吸附测定法（ELISA），用于检测针对纯化的爱泼斯坦-巴尔病毒（EBV）多肽的IgA抗体。该三步法包括使用小鼠抗人IgA单克隆抗体（MAb）来放大IgA反应。该测定中使用的两种主要EBV蛋白是与病毒衣壳抗原（VCA）复合物相关的125 kDa成分（gp125）和与膜抗原（MA）复合物相关的一种主要糖蛋白（gp250/200）。在含有这两种糖蛋白或分别含有每种糖蛋白的ELISA板上检测了82份血清。其中包括45份来自鼻咽癌（NPC）患者的IgA抗体阳性血清。对于这些血清，免疫荧光（IF）和ELISA方法的结果在定性和定量上都有良好的相关性。虽然大多数IgA抗体阳性血清含有与gp125和gp250/200都反应的抗体，但也有一些血清只含有与其中一种糖蛋白反应而不与另一种反应的抗体。数据表明，在检测针对EBV的IgA抗体的测定中应同时使用这两种糖蛋白，以避免假阴性结果。该测定法对于在大量人群中筛查针对EBV的IgA抗体以及可能监测NPC患者的病程应该是有用的。
