Zhao Yangyang, Zhao Jing, Shan Guiye, Yan Dongmei, Chen Yanwei, Liu Yichun
Centre for Advanced Optoelectronic Functional Materials Research, Key Laboratory for UV Light-Emitting Materials and Technology of the Ministry of Education, Northeast Normal University, Changchun, 130024, China.
Centre for Advanced Optoelectronic Functional Materials Research, Key Laboratory for UV Light-Emitting Materials and Technology of the Ministry of Education, Northeast Normal University, Changchun, 130024, China.
Colloids Surf B Biointerfaces. 2017 Jun 1;154:150-159. doi: 10.1016/j.colsurfb.2017.03.016. Epub 2017 Mar 8.
It is important to control drug release and detect the distribution of drug molecules before and after release. In this work, liposome@AgAu nanocomposite is prepared for drug delivery, which not only can control drug release by near infrared laser irradiation but also can monitor drug molecules by surface enhanced Raman scattering (SERS) and fluorescence signal during the release process. The liposome@AgAu core/shell nanocomposite prepared by the galvanic replacement reaction (GRR) shows tunable localized surface plasmon resonance (LSPR) absorption peaks from visible to near-infrared region and good biocompatibility. Comparing to pure doxorubicin (DOX) molecules, liposome@AgAu nanocomposite loading DOX exhibit lower cytotoxicity by MTT assay. After loading into liposome@AgAu, the fluorescence signal of DOX disappear due to the fluorescence resonance energy transfer from DOX to metals shell. On the contrary, the SERS signal of DOX in liposome@AgAu is obviously increased. Furthermore, the liposome@AgAu nanocomposite shows photothermal conversion ability under resonance laser irradiation. Under 633nm laser irradiation, the liposome@AgAu nanocomposite loading DOX can release drug molecules for killing cancer cell. Meanwhile, the fluorescence signal from DOX appears after drug release from liposome@AgAu, but the SERS signal is not obvious. Therefore, this nanocomposite can provide a platform for photothermal controllable drug release and optical signal targeting for drug molecules.
控制药物释放并检测释放前后药物分子的分布非常重要。在这项工作中，制备了用于药物递送的脂质体@AgAu纳米复合材料，其不仅可以通过近红外激光照射来控制药物释放，还可以在释放过程中通过表面增强拉曼散射（SERS）和荧光信号监测药物分子。通过电置换反应（GRR）制备的脂质体@AgAu核壳纳米复合材料在可见光到近红外区域显示出可调谐的局域表面等离子体共振（LSPR）吸收峰以及良好的生物相容性。与纯阿霉素（DOX）分子相比，负载DOX的脂质体@AgAu纳米复合材料通过MTT法显示出较低的细胞毒性。负载到脂质体@AgAu中后，由于从DOX到金属壳的荧光共振能量转移，DOX的荧光信号消失。相反，脂质体@AgAu中DOX的SERS信号明显增强。此外，脂质体@AgAu纳米复合材料在共振激光照射下显示出光热转换能力。在633nm激光照射下，负载DOX的脂质体@AgAu纳米复合材料可以释放药物分子以杀死癌细胞。同时，从脂质体@AgAu释放药物后，DOX的荧光信号出现，但SERS信号不明显。因此，这种纳米复合材料可以为光热可控药物释放和药物分子的光信号靶向提供一个平台。
