Tarpani Luigi, Morena Francesco, Gambucci Marta, Zampini Giulia, Massaro Giuseppina, Argentati Chiara, Emiliani Carla, Martino Sabata, Latterini Loredana
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto, 8, 06123 Perugia, Italy.
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06123 Perugia, Italy.
Nanomaterials (Basel). 2016 Jun 4;6(6):104. doi: 10.3390/nano6060104.
The preparation of tailored nanomaterials able to support cell growth and viability is mandatory for tissue engineering applications. In the present work, silica nanoparticles were prepared by a sol-gel procedure and were then functionalized by condensation of amino groups and by adsorption of silver nanoparticles. Transmission electron microscopy (TEM) imaging was used to establish the morphology and the average dimensions of about 130 nm, which were not affected by the functionalization. The three silica samples were deposited (1 mg/mL) on cover glasses, which were used as a substrate to culture adult human bone marrow-mesenchymal stem cells (hBM-MSCs) and human adipose-derived stem cells (hASCs). The good cell viability over the different silica surfaces was evaluated by monitoring the mitochondrial dehydrogenase activity. The analysis of the morphological parameters (aspect ratio, cell length, and nuclear shape Index) yielded information about the interactions of stem cells with the surface of three different nanoparticles. The data are discussed in terms of chemical properties of the surface of silica nanoparticles.
制备能够支持细胞生长和活力的定制纳米材料对于组织工程应用来说是必不可少的。在本工作中，通过溶胶-凝胶法制备了二氧化硅纳米颗粒，然后通过氨基缩合和银纳米颗粒吸附对其进行功能化。利用透射电子显微镜（TEM）成像确定了其形态以及约130 nm的平均尺寸，这些尺寸不受功能化影响。将三种二氧化硅样品（1 mg/mL）沉积在盖玻片上，盖玻片用作培养成人骨髓间充质干细胞（hBM-MSCs）和人脂肪来源干细胞（hASCs）的基质。通过监测线粒体脱氢酶活性评估了不同二氧化硅表面上良好的细胞活力。对形态学参数（纵横比、细胞长度和核形状指数）的分析得出了有关干细胞与三种不同纳米颗粒表面相互作用的信息。根据二氧化硅纳米颗粒表面的化学性质对数据进行了讨论。
