Cordeiro Milton, Carvalho Lara, Silva Joana, Saúde Leonor, Fernandes Alexandra R, Baptista Pedro V
UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Nanomaterials (Basel). 2017 Jan 11;7(1):10. doi: 10.3390/nano7010010.
The use of gold nanoparticles for effective gene silencing has demonstrated its potential as a tool for gene expression experiments and for the treatment of several diseases. Here, we used a gold nanobeacon designed to specifically silence the enhanced green fluorescence protein (EGFP) mRNA in embryos of a fli-EGFP transgenic zebrafish line, while simultaneously allowing the tracking and localization of the silencing events via the beacon's emission. Fluorescence imaging measurements demonstrated a decrease of the EGFP emission with a concomitant increase in the fluorescence of the Au-nanobeacon. Furthermore, microinjection of the Au-nanobeacon led to a negligible difference in mortality and malformations in comparison to the free oligonucleotide, indicating that this system is a biocompatible platform for the administration of gene silencing moieties. Together, these data illustrate the potential of Au-nanobeacons as tools for in vivo zebrafish gene modulation with low toxicity which may be used towards any gene of interest.
使用金纳米颗粒进行有效的基因沉默已证明其作为基因表达实验工具和治疗多种疾病的潜力。在此，我们使用了一种金纳米信标，其设计目的是在fli-EGFP转基因斑马鱼品系的胚胎中特异性沉默增强型绿色荧光蛋白（EGFP）mRNA，同时通过信标的发射允许对沉默事件进行跟踪和定位。荧光成像测量表明，EGFP发射减少，同时金纳米信标的荧光增加。此外，与游离寡核苷酸相比，金纳米信标的显微注射导致死亡率和畸形率的差异可忽略不计，这表明该系统是用于给药基因沉默部分的生物相容性平台。总之，这些数据说明了金纳米信标作为体内斑马鱼基因调节工具的潜力，其毒性低，可用于任何感兴趣的基因。
