濒危非洲特有树种波伊尔的微卫星标记的分离与鉴定
Isolation and characterization of microsatellite markers for the threatened African endemic tree species Poir.
作者信息
Johnson Benziwa Nathalie, Quashie Marie Luce Akossiwoa, Chaix Gilles, Camus-Kulandaivelu Letizia, Adjonou Kossi, Segla Kossi Novinyo, Kokutse Adzo Dzifa, Ouinsavi Christine, Bationo Babou André, Rabiou Habou, Kokou Kouami, Vignes Hélène
机构信息
Laboratoire de Recherche Forestière (LRF) Université de Lomé Lomé Togo.
CIRAD, UMR AGAP Montpellier France.
出版信息
Ecol Evol. 2020 Oct 27;10(23):13403-13411. doi: 10.1002/ece3.6944. eCollection 2020 Dec.
To study the genetic diversity and structure of the forest species Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using next-generation sequencing. Three hundred and sixty-five (365) individuals were analyzed within fifteen (15) West African populations. The number of alleles for these loci varied from 4 to 30, and the heterozygosity varied from 0.23 to 0.82. The seventeen (17) primers designed here will allow characterizing the genetic diversity of this threaten species on its natural stands and to better understand the population differentiation mechanisms shaping it.
为了研究森林物种Poir.的遗传多样性和结构，利用下一代测序技术分离并鉴定了17个多态性核微卫星标记。对15个西非种群中的365个个体进行了分析。这些位点的等位基因数量从4个到30个不等，杂合度从0.23到0.82不等。在此设计的17个引物将有助于在其自然林分中表征这种濒危物种的遗传多样性，并更好地理解塑造它的种群分化机制。
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