Jiao Si-Qian, Sun Yan-Qiang, Zhang Dong-Xu, Gao Qiong, Jin Yuqing, Liu Hui, Ma Yongpeng, Yang Yong, Porth Ilga, Mao Jian-Feng
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design National Engineering Laboratory for Tree Breeding Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of Education College of Biological Sciences and Technology Beijing Forestry University Beijing 100083 People's Republic of China.
College of Life Science Datong University Datong 037009 Shanxi People's Republic of China.
Appl Plant Sci. 2019 Jan 16;7(1):e01212. doi: 10.1002/aps3.1212. eCollection 2019 Jan.
(Ephedraceae) is a gymnosperm shrub with a wide distribution across Central and Eastern Asia. It is widely cultivated as a medicinal plant, but its wild populations are monitored to determine whether protection is needed.
Thirty-six microsatellite markers, including 11 polymorphic markers, were developed from RNA-Seq data deposited in the National Center for Biotechology Information dbEST database. Among 100 genotyped individuals originating from five different population groups, the allele number ranged from three to 22 per locus. Levels of observed and expected heterozygosity ranged from 0 to 0.866 (average 0.176) and 0 to 0.876 (average 0.491), respectively. Allelic polymorphism information content ranged from 0.000 to 0.847 (average 0.333). Cross-species amplifications were successfully conducted with two related species for all 11 di- or trinucleotide simple sequence repeats.
This study provides the first set of microsatellite markers for genetic monitoring and surveying of this medicinal plant.
麻黄科植物是一种裸子植物灌木，广泛分布于中亚和东亚地区。它作为药用植物被广泛种植，但其野生种群需要监测以确定是否需要保护。
从美国国立生物技术信息中心dbEST数据库中存储的RNA测序数据开发了36个微卫星标记，其中包括11个多态性标记。在来自五个不同种群组的100个基因分型个体中，每个位点的等位基因数从3到22不等。观察到的杂合度和预期杂合度水平分别为0至0.866（平均0.176）和0至0.876（平均0.491）。等位基因多态性信息含量范围为0.000至0.847（平均0.333）。对于所有11个二核苷酸或三核苷酸简单序列重复，成功地在两个相关物种中进行了跨物种扩增。
本研究为这种药用植物的遗传监测和调查提供了第一组微卫星标记。
