Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Institute of Physics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Sci Rep. 2017 Mar 24;7:45183. doi: 10.1038/srep45183.
Non-thermal plasma (NTP) has nonspecific antibacterial effects, and can be applied as an effective tool for the treatment of chronic wounds and other skin pathologies. In this study we analysed the effect of NTP on the healing of the full-thickness acute skin wound model in rats. We utilised a single jet NTP system generating atmospheric pressure air plasma, with ion volume density 5 · 10 m and gas temperature 30-35 °C. The skin wounds were exposed to three daily plasma treatments for 1 or 2 minutes and were evaluated 3, 7 and 14 days after the wounding by histological and gene expression analysis. NTP treatment significantly enhanced epithelization and wound contraction on day 7 when compared to the untreated wounds. Macrophage infiltration into the wound area was not affected by the NTP treatment. Gene expression analysis did not indicate an increased inflammatory reaction or a disruption of the wound healing process; transient enhancement of inflammatory marker upregulation was found after NTP treatment on day 7. In summary, NTP treatment had improved the healing efficacy of acute skin wounds without noticeable side effects and concomitant activation of pro-inflammatory signalling. The obtained results highlight the favourability of plasma applications for wound therapy in clinics.
非热等离子体（NTP）具有非特异性的抗菌作用，可作为治疗慢性创面和其他皮肤病变的有效工具。本研究分析了 NTP 对大鼠全层急性皮肤创面模型愈合的影响。我们使用单射流 NTP 系统产生大气压空气等离子体，离子体积密度为 5×10^16m^−3，气体温度为 30-35°C。皮肤创面每天接受三次等离子体处理，每次 1 或 2 分钟，并在创伤后 3、7 和 14 天进行组织学和基因表达分析。与未处理的创面相比，NTP 处理在第 7 天显著促进了上皮化和创面收缩。NTP 处理对巨噬细胞浸润到创面区域没有影响。基因表达分析表明，NTP 处理后第 7 天炎症反应增强或创面愈合过程中断；NTP 处理后发现炎症标志物的短暂上调增强。总之，NTP 处理改善了急性皮肤创面的愈合效果，没有明显的副作用和伴随的促炎信号激活。获得的结果强调了等离子体应用于临床创面治疗的有利性。
