School of Life Science, Shanxi Normal University, Linfen City, 041004, China.
School of Food Science, Shanxi Normal University, Linfen City, 041004, China.
Sci Rep. 2017 Mar 24;7:45231. doi: 10.1038/srep45231.
Cyperus rotundus L. (Cyperaceae) is a medicinal herb traditionally used to treat various clinical conditions at home. In this study, chemical composition of Cyperus rotundus rhizomes essential oil, and in vitro antioxidant, DNA damage protective and cytotoxic activities as well as antibacterial activity against foodborne pathogens were investigated. Results showed that α-cyperone (38.46%), cyperene (12.84%) and α-selinene (11.66%) were the major components of the essential oil. The essential oil had an excellent antioxidant activity, the protective effect against DNA damage, and cytotoxic effects on the human neuroblastoma SH-SY5Y cell, as well as antibacterial activity against several foodborne pathogens. These biological activities were dose-dependent, increasing with higher dosage in a certain concentration range. The antibacterial effects of essential oil were greater against Gram-positive bacteria as compared to Gram-negative bacteria, and the antibacterial effects were significantly influenced by incubation time and concentration. These results may provide biological evidence for the practical application of the C. rotundus rhizomes essential oil in food and pharmaceutical industries.
香附（Cyperus rotundus L.）（莎草科）是一种药用草本植物，传统上在家中用于治疗各种临床病症。在这项研究中，对香附根茎精油的化学成分、体外抗氧化、DNA 损伤保护、细胞毒性活性以及对食源性致病菌的抗菌活性进行了研究。结果表明，α-环高蒈酮（38.46%）、环高蒈烯（12.84%）和α-瑟林烯（11.66%）是精油的主要成分。该精油具有出色的抗氧化活性、对 DNA 损伤的保护作用、对人神经母细胞瘤 SH-SY5Y 细胞的细胞毒性作用以及对几种食源性致病菌的抗菌活性。这些生物活性呈剂量依赖性，在一定浓度范围内，随着剂量的增加而增加。与革兰氏阴性菌相比，精油对革兰氏阳性菌的抗菌作用更大，并且抗菌效果受孵育时间和浓度的显著影响。这些结果可能为香附根茎精油在食品和制药工业中的实际应用提供生物学依据。
