Saito Teruaki, Sakuta Genki, Kobayashi Hitoshi, Ouchi Kenji, Inatomi Satoshi
Mushroom Research Laboratory, Hokuto Corporation, Nagano, Japan.
Mycobiology. 2019 Oct 3;47(4):466-472. doi: 10.1080/12298093.2019.1665331. eCollection 2019.
For the purpose of protecting the rights of breeders, we developed a new simple sequence repeat (SSR) marker set consisting only of genetically independent tetranucleotide or longer core motifs. Using available genome sequences for five strains, we designed primers for 13 SSR markers that amplified polymorphic sequences in 20 cultivars. We evaluated the independence of every possible marker pair based on genotype data. Consequently, eight genetically independent markers were selected. The polymorphic information content values of the markers ranged from 0.269 to 0.764, with an average of 0.409. The markers could distinguish among 20 cultivars and produced highly repeatable and reproducible results. The markers developed in this study will enable the precise identification of cultivars, and may be useful for protecting breeders' rights.
为了保护育种者的权益，我们开发了一种新的简单序列重复（SSR）标记集，该标记集仅由遗传上独立的四核苷酸或更长的核心基序组成。利用五个菌株的可用基因组序列，我们设计了13个SSR标记的引物，这些引物在20个品种中扩增出多态性序列。我们根据基因型数据评估了每对可能的标记的独立性。结果，选择了八个遗传上独立的标记。这些标记的多态信息含量值范围为0.269至0.764，平均为0.409。这些标记可以区分20个品种，并产生高度可重复和可再现的结果。本研究中开发的标记将能够精确鉴定品种，并可能有助于保护育种者的权益。
