Osimani Andrea, Garofalo Cristiana, Aquilanti Lucia, Milanović Vesna, Cardinali Federica, Taccari Manuela, Pasquini Marina, Tavoletti Stefano, Clementi Francesca
Dipartimento di Scienze Agrarie, Alimentari ed Ambientali, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
J Food Sci. 2017 May;82(5):1184-1192. doi: 10.1111/1750-3841.13700. Epub 2017 Mar 24.
Grasshoppers are the most commonly eaten insects by humans worldwide, as they are rich in proteins and micronutrients. This study aimed to assess the occurrence of transferable antibiotic resistance genes in commercialized edible grasshoppers. To this end, the prevalence of 12 selected genes [aac(6')-Ie aph(2″)-Ia, blaZ, erm(A), erm(B), erm(C), mecA, tet(M), tet(O), tet(S), tet(K), vanA, vanB] coding for resistance to antibiotics conventionally used in clinical practice was determined. The majority of samples were positive for tet(M) (70.0%), tet(K) (83.3%) and blaZ (83.3%). A low percentage of samples were positive for erm(B) (16.7%), erm(C) (26.7%), and aac(6')-Ie aph(2″)-Ia (13.3%), whereas no samples were positive for erm(A), vanA, vanB, tet(O), and mecA. Cluster analysis identified 4 main clusters, allowing a separation of samples on the basis of their country of origin.
蝗虫是全球人类最常食用的昆虫，因为它们富含蛋白质和微量营养素。本研究旨在评估商业化食用蝗虫中可转移抗生素抗性基因的存在情况。为此，测定了12个选定基因[aac(6')-Ie aph(2″)-Ia、blaZ、erm(A)、erm(B)、erm(C)、mecA、tet(M)、tet(O)、tet(S)、tet(K)、vanA、vanB]的流行情况，这些基因编码对临床实践中常用抗生素的抗性。大多数样本tet(M)（70.0%）、tet(K)（83.3%）和blaZ（83.3%）呈阳性。低比例样本erm(B)（16.7%）、erm(C)（26.7%）和aac(6')-Ie aph(2″)-Ia（13.3%）呈阳性，而没有样本erm(A)、vanA、vanB、tet(O)和mecA呈阳性。聚类分析确定了4个主要聚类，能够根据样本的原产国对其进行区分。
